Words similar to double-strand
Example sentences for: double-strand
How can you use “double-strand” in a sentence? Here are some example sentences to help you improve your vocabulary:
The first round combined reverse transcription using oligo-dT (15)-T7 (5'-AAACGACGGCCAGTGAATTGTAATACGACTCACTATAGGCGC-3') primer and template-switch primer (5'-AAGCAGTGGTATCAACGCAGAGTACGCGGG-3') (Clontech, Palo Alto, CA) catalyzed by the Superscript-11 reverse transcriptase (Gibco-BRL) and Advantage cDNA polymerase (Clontech) for single- and double-strand cDNA synthesis respectively, with in vitro transcription (Megascript kit, Ambion, Austin, TX).
Double-strand (ds) breakage of chromosomal DNA is obviously a serious threat to cells because various activities of the chromosome - gene expression, replication and partition - depend on its integrity.
Using a double-strand RNA interference (dsRNAi) technique designed to eliminate specific proteins [ 15 16 ] , such as Arr, DFz2 and Dsh, we have developed strong evidence that Wg requires both types of receptors to transduce a signal efficiently through Dsh to stabilize β-catenin.
Rad52 functions in conjunction with the RecA ortholog, the RAD51 recombinase in double-strand break repair and meiotic recombination [ 6 ] . RAD52 binds ssDNA during recombination and also shows a quaternary organization similar to those of RecT/Redβ and ERF [ 16 26 ] . However, RAD52-like proteins showed no detectable sequence similarity with either the ERF or the RecT/Redβ-like proteins.
If a chromosome carries multiple replication forks as usual at 37°C in rich media, more than one double-strand break may be necessary to form a non-circular, branched species, which should be able to move through the gel.
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