Example sentences for: dot-blot

How can you use “dot-blot” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • A dot-blot hybridization assay was performed using DNA from B. thuringiensis strain AWO6 as probe against PCR-amplified pXO2 ORF DNA applied to a nylon membrane.

  • The strength of each probe was determined by a dot-blot color reaction (Figure 1b).

  • GAG detection was also performed by the Toluidine Blue-O precipitation and dot-blot method as described previously [ 34 ] . Briefly, 200 μl of sample were applied to each well of a 48-well manifold (Life Technologies) onto a PVDF membrane without vacuum.

  • We determined the success of each hybridization experiment by taking into account the results of the agarose gel analysis of PCR products, dot-blot analysis of probes, microarray data (described below), available information from public databases, and the quality of the captured images.

  • BDNF exon-containing subclones were identified by dot-blot hybridization using digoxigenin-labelled probes prepared from 5' RACE clones by PCR.


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