Words similar to dna-dependent
Example sentences for: dna-dependent
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The RDRPs of RNA viruses define one major lineage of nucleic acid polymerases, which additionally includes reverse transcriptases, archaeo-eukaryotic DNA polymerases, and nucleotide cyclases [ 8 9 10 11 12 13 ] . The DNA-dependent RNA polymerase of certain bacteriophages, such as T7, and the archaeo-eukaryotic primase (also detected in some bacteria) are divergent derivatives of the same fold [ 11 14 ] . The core catalytic domain of all these enzymes, the so-called "palm" domain, has an RNA-recognition motif (RRM)-like fold with strategically placed metal-coordinating residues, which form the active site [ 11 15 16 ] . In contrast, bacterial DnaG-type primases (also present in archaea and some eukaryotes) contain a polymerase domain of the Rossmann-like TOPRIM fold, which is shared with topoisomerases and OLD-family nucleases [ 17 18 19 ] . The recently solved structures of the DDRPs from yeast and the thermophilic bacterium Thermus thermophilus indicate that the β' subunit (according to the subunit nomenclature of Escherichia coli DDRP, which we hereinafter employ to designate all orthologs of the respective E. coli subunits) of these enzymes defines another distinct catalytic scaffold, which is unrelated to any of the above template-dependent RNA polymerases [ 20 21 22 23 24 ] . Additionally, the structural and evolutionary affinities of two other template-dependent RNA polymerases, namely RDRPs involved in PTGS [ 25 26 27 ] and primases of herpesviruses [ 28 ] , remain obscure.
The purified protein has DNA-dependent ATPase activity and DNA unwinding activity (Figure 1C& 1D) equivalent to previous preparations without GFP expressed in S. cerevisiae cells [ 10].
GFP-BLM has 5 units/ng unwinding activity and 10 units/ng DNA-dependent ATPase activity as compared to 6 units/ng and 7 units/ng for BLM purified from yeast.
Other PIKK family members also function to orchestrate eukaryotic genotoxic stress responses, including human ATM, ATR, and DNA-dependent protein kinase (DNA-PKcs), TEL1 and MEC1 of Saccharomyces cerevisiae , Mei-41 of Drosophila and UVSB of Aspergillus nidulans [ 5 32 33 34 ] .
Ancient conserved domains and evolution of DNA-dependent RNA polymerases
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