Example sentences for: dithiothreitol

How can you use “dithiothreitol” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • All purification steps contained dithiothreitol to prevent oxidation of the proteins.

  • Reverse transcription was performed in the presence of 500 μM each of dATP, dCTP, and dGTP, 200 μM 5-aminoallyl-dUTP (Sigma), 300 μM dTTP, 1 × first-strand buffer, 10 mM dithiothreitol, and 400 U Superscript II (Life Technologies) in 40-μl reactions at 42°C for 3 h to overnight.

  • Protein in positive elution fractions was refolded to native structure by serial dialysis against buffer D (20 mm HEPES, 0.1 M KCl, 0.2 mm EDTA, 0.5 mm dithiothreitol, 0.5 mm phenylmethylsulfonyl fluoride, 20% glycerol) containing stepwise decreasing concentrations of urea (6 M for 2 hours, 4 M for 2 hours, 2 M for 2 hours, no urea for 12 hours).

  • 9, 10 mM Tris-HCl pH 7.5, 100 mM NaCl, 15 mM KCl, 0.5 mM EDTA, 1 mM dithiothreitol, 10% glycerol, and 2 μg dIdC.

  • Samples containing 60 μg of total protein were mixed with an equal volume of 2x SDS-Laemmli sample buffer (100 mM Tris-HCl, pH 6.8, 20% glycerol, 200 mM dithiothreitol, 4% SDS, and 0.2% bromphenol blue) and boiled for 5 min, then resolved using either 12% SDS-polyacrylamide gel electrophoresis.

How many words do you know? Try our free vocabulary size test!


Search for example sentences

Loading Loading...