Words similar to dithiothreitol

Example sentences for: dithiothreitol

How can you use “dithiothreitol” in a sentence? Here are some example sentences to help you improve your vocabulary:

Cells were then resuspended in buffer A (50 mM Tris-HCl pH 7.5, 20% glycerol, 1 mM Dithiothreitol, 0.2% Nonidet P-40) with 0. 5 M KCl and subjected to two rounds of freeze-thaw followed by a 30 min incubation on ice.
An equal mass of each protein sample was subjected to reducing conditions (99°C for 5 min in 100 mM dithiothreitol (DTT)), separated on a 15% SDS-PAGE, and transferred to nitrocellulose membrane according to standard protocols.
Cell monolayers were washed with PBS three times and then lysed in lysis buffer (50 mM Tris-HCl, pH 7.5, 150 mM KCl, 1 mM dithiothreitol, 1 mM EDTA, 10 μg/ml leupeptin, 2 μg/ml Aprotinin, and 0.1 mM PMSF) by subjecting the cells to 3 cycles of freezing in liquid nitrogen and thawing in a 37°C water bath.
5 mM mgCl2, 10 mM Hepes, pH 6.8, 0.1 mM dithiothreitol, 10 ug/ml cycloheximide) and centrifuged at 4°C in a SW50.
The purified 5 μg aRNA was labeled with Cy3 or Cy5 by reverse transcription in a solution containing 10 μg of random hexamer, 4 μl first-strand reaction buffer, 2 μl 10 mM dithiothreitol (DTT; Gibco-BRL), 1 μl of 2 mM dATP, dGTP, dTTP and 1 mM dCTP, 1 μl SUPERase-in, (Amtion) and 200 u Superscript II reverse transcriptase (Gibco-BRL).