Words similar to dithiothreitol

Example sentences for: dithiothreitol

How can you use “dithiothreitol” in a sentence? Here are some example sentences to help you improve your vocabulary:

Swine carotid arteries were first thermally and histamine stimulated, followed by freezing in an acetone-dry ice slurry [ 15 ] . After air drying, the tissues were homogenized in a buffer (20 mg wet weight tissue/ml buffer) containing 1% SDS, 10% glycerol, and 1 mM dithiothreitol.
The cell pellet was washed once in cold phosphate-buffered saline and subsequently resuspended in hypotonic lysis buffer (10 mM Tris.Cl, pH 7.9, 10 mM KCl, 1.5 mM MgCl2, 1 mM phenylmethylsulfonyl fluoride, 2 μg/ml pepstatin, 2 μg/ml leupeptin, 5 μg/ml aprotinin, 1 mM dithiothreitol).
The purified 5 μg aRNA was labeled with Cy3 or Cy5 by reverse transcription in a solution containing 10 μg of random hexamer, 4 μl first-strand reaction buffer, 2 μl 10 mM dithiothreitol (DTT; Gibco-BRL), 1 μl of 2 mM dATP, dGTP, dTTP and 1 mM dCTP, 1 μl SUPERase-in, (Amtion) and 200 u Superscript II reverse transcriptase (Gibco-BRL).
1 M sodium pyrophosphate, pH 8.4, 1.0 mM EDTA, 5.0 mM dithiothreitol or glutathione); 1.0 mM NAD.
Cell monolayers were washed with PBS three times and then lysed in lysis buffer (50 mM Tris-HCl, pH 7.5, 150 mM KCl, 1 mM dithiothreitol, 1 mM EDTA, 10 μg/ml leupeptin, 2 μg/ml Aprotinin, and 0.1 mM PMSF) by subjecting the cells to 3 cycles of freezing in liquid nitrogen and thawing in a 37°C water bath.