Example sentences for: ditags

How can you use “ditags” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The fact that the tag abundance levels differ slightly more in the libraries made from the same ditag ligation than in those made from the same mRNA sample may reflect a small amount of denaturation of AT-rich ditags during storage of the ligation reaction, as has been previously reported [ 24 ] . Overall, microSAGE passes all the tests for representativity, reproducibility and accuracy used previously for conventional SAGE [ 2 ] .

  • Although the amount of starting material used to generate each of the libraries varied somewhat, we did not find any substantial changes in the fraction of duplicate ditags found in each library, which we have found to be more or less constant (approximately 5%) when more than 1 μg total RNA is used (S.B.

  • Six nanograms of each linker was used to generate the ditags and 28 cycles of amplification were used to obtain ditags from each of the samples, with the exception of the E12.

  • We made libraries from the same mRNA samples to examine the variability due to library construction, and made sublibraries from identical pools of ditags to examine the variability due to the last steps in SAGE library construction, as well as due to sampling.

  • The present analysis is based on 76,280 15-mer tags from NGF-untreated PC12 cells and 87,004 tags from NGF-treated cells (after exclusion of duplicate ditags, mitochondrial transcripts and repetitive elements).


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