Words similar to ditag
Example sentences for: ditag
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5 retinal libraries made from the same ditag ligation, but different large-scale PCR preparations, showed a correlation coefficient of 0.938 when all tags were considered, little difference between observed and expected p -values of variance (Figure 4), and showed relatively little difference between abundant tags (Figure 5b).
5B (library construction from ditag stage); Additional data file 3- crx +/+A vs crx +/+B (library construction from mRNA stage); Additional data file 4- 41-year-old peripheral retina and 44-year-old peripheral retina (individual/environmental variation); Additional data file 5- 44-year-old peripheral retina and 88-year-old peripheral retina (individual variation/age- and sex-dependent gene expression); Additional data file 6- P6.
5B SAGE library, ditag amplification took place after the initial ditag ligation had been stored at -20°C for 3 months.
Likewise, tag abundance levels in libraries made from identical mRNA samples and ditag ligations showed very high similarity.
The fact that the tag abundance levels differ slightly more in the libraries made from the same ditag ligation than in those made from the same mRNA sample may reflect a small amount of denaturation of AT-rich ditags during storage of the ligation reaction, as has been previously reported [ 24 ] . Overall, microSAGE passes all the tests for representativity, reproducibility and accuracy used previously for conventional SAGE [ 2 ] .