Example sentences for: diphenyltetrazolium

How can you use “diphenyltetrazolium” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Cells were plated in 96-well plates (human primary RPE: 10,000 cells/well, ARPE-19: 15,000-20,000 cells/well, unless otherwise stated) overnight, fed with serum-free medium or treated with testing agents (prepared in serum-free medium) the next day followed by H 2 O 2 treatment for a day, then the viability from each treatment was determined by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay [ 27 ] . Culture medium was removed after treatment and then 100 μl MTT solution (100 μg/ml prepared in DMEM) was added to each well.

  • 15d-PGJ 2 , 15-deoxy-delta 12, 14-Prostaglandin J 2 ; ERK, Extracellular signal-Regulated Kinase; 4-HNE, 4-hydroxynonenal, MAP kinases, Mitogen-Activated Protein kinases; MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide); PBS, phosphate-buffered saline; PPARs, peroxisome proliferator-activated receptors; RPE, retinal pigment epithelium.

  • Cells grown in 96-well plates (20,000 cells/well, ~90% confluency) were treated with testing agents for a period of time as indicated in the figure legends; then, the viability from each treatment was determined by the MTT (3-(4,5-dimethylthiazole-2yl)-2,5-diphenyltetrazolium bromide) assay [ 66 ] . Culture medium was removed after treatment, then 100 μl MTT solution (100 μg/ml prepared in MEM) was added to each well.

  • DRB, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and ionomycin were purchased from Sigma.

  • This assay for cell viability is based on the reduction of 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) by mitochondrial dehydrogenase in viable cells to produce a purple formazan product.


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