Words similar to dinucleoside
Example sentences for: dinucleoside
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An earlier report demonstrated that disruption of HNT2 was tolerated by haploid yeast strains without an effect on growth and that ApppN and AppppN accumulate 30 and 3-fold, respectively on account of the hnt2 deletion [ 13 ] . Because those data were obtained by random spore analysis, we considered it important to test whether elevated dinucleoside polyphosphate levels co-segregate with hnt2 disruption in all tetrads examined and whether any other commonly used genetic markers affect dinucleoside polyphosphate levels.
This reduction was active-site dependent: adding back multicopy HNT2 with the nucleophilic histidine replaced by alanine or aspartate reduced dinucleoside polyphosphate levels less than two-fold.
Catalytic activity of the Fhit ApppA hydrolase depends on His96 [ 14 16 ] . To test whether the active site of Hnt2 is necessary to control dinucleoside polyphosphate accumulation in vivo , wild-type and mutant alleles of HNT2 that differ at His109, corresponding to human Fhit His96, were expressed from the HNT2 promoter on plasmids (Table 3) in hnt2ΔADE2 strain BY71-6c.
While cells without a functional HNT2 gene accumulate dinucleoside polyphosphates in excess of 10 μM in a variety of nonstressed and stressed conditions, 46°C heat shock was the only condition that produced dinucleoside polyphosphate accumulation in excess of 10 μM in cells containing a functional HNT2 gene.
Heat shock is the most effective stress for elevation of dinucleoside polyphosphates