Words similar to dinucleoside
Example sentences for: dinucleoside
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Increases in dinucleoside polyphosphates in Hnt2+ cultures cannot be attributed to thermal inactivation of Hnt2 because the presence of HNT2 plasmids continues to reduce incremental increases in dinucleoside polyphosphates even in the fourth hour of the heat shock.
An earlier report demonstrated that disruption of HNT2 was tolerated by haploid yeast strains without an effect on growth and that ApppN and AppppN accumulate 30 and 3-fold, respectively on account of the hnt2 deletion [ 13 ] . Because those data were obtained by random spore analysis, we considered it important to test whether elevated dinucleoside polyphosphate levels co-segregate with hnt2 disruption in all tetrads examined and whether any other commonly used genetic markers affect dinucleoside polyphosphate levels.
Catalytic activity of the Fhit ApppA hydrolase depends on His96 [ 14 16 ] . To test whether the active site of Hnt2 is necessary to control dinucleoside polyphosphate accumulation in vivo , wild-type and mutant alleles of HNT2 that differ at His109, corresponding to human Fhit His96, were expressed from the HNT2 promoter on plasmids (Table 3) in hnt2ΔADE2 strain BY71-6c.
In the case of disruption of the Fhit and Hnt2-homologous aph1 gene in S. pombe , which encodes an enzyme relatively specific for a AppppA [ 20 ] , a 290-fold increase in AppppA concentration was observed [ 22 ] . Our data indicate that Hnt2 hydrolyzes ApppN and AppppN in vivo in budding yeast and that an intact adenine biosynthetic pathway is required for high-level synthesis and accumulation of adenylylated dinucleoside polyphosphates.
Dinucleoside polyphosphate levels may not be limited by the levels of lysyl tRNA synthetase
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