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Example sentences for: dilutions

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The same crosses were also used to determine if Tab +was recessive or dominant: for every tab mutant, haploid cdc15Δ tab [pMET3-cdcl5-2, URA3] and diploid cdc15Δ/cdc15Δ tab/+ [pMET3-cdc15-2, URA3 ] cells were pre-grown on YPD (1% yeast extract/2% peptone + 2% glucose) at 25°C for 2-3 days, spotted in 5-fold serial dilutions onto SD+5-FOA plates, and the number of 5-FOA resistant colonies from the diploid was divided by that from the haploid to obtain ratio P. The mutant was defined to be dominant if P= 0.1-1, semi-dominant if P= 0.01-0.
Standard curves for each amplification product were generated from 10-fold dilutions of pooled cDNA to determine primer efficiency.
After 3 washes in PBS, the sections were incubated with the corresponding biotinylated secondary antibody (Vector Laboratories, Inc, CA) at 1:200 dilutions for 1 hour at room temperature followed by ABC-DAB method (Vector Laboratories, Inc) according to the manufacturer's protocol.
Individual AV DNA titers were determined by three different methods: 1) plaque titration on 293 cells, 2) immunofluorescence microscopy of AV protein expression (anti-penton group antigen, clone 143, Biodesign, Kennebunk, ME) in 293 cells infected with serial dilutions of AV and 3) absorbance at 260 nm (pfu/ml = A
Individual assay sensitivity was analyzed by the use of serial dilutions of SiHa cell line crude cell digest, targeting 10 4, 10 3, 10 2, 10 1, and 10 0input copies of HPV-16.