Example sentences for: diluted

How can you use “diluted” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 10 μl of the brush border membrane preparation was diluted in 90 μl of Laemmli buffer, boiled for 5 min and separated on a denaturing polyacrylamide gel (5 % stacking gel/10 % resolving gel) at 120 V over 2 hours [ 18 ] . The separated proteins were transferred onto nitrocellulose membrane using a Trans-blot instrument (Biorad, Hercules, CA) in glycine-methanol buffer following the manufacturer's suggestions.

  • In general, PCR reactions were performed in a volume of 50 μl with 50 picomoles of each primer, dNTP's (Amersham Pharmacia Biotech, Inc., Piscataway, NJ) at a final concentration of 0.5 mM, 1 μl of template, 1X PCR buffer (10X PCR buffer contained 700 mM Tris pH 8.8, 20 mM MgCl 2 , 1% Triton X-100, and 0.1% Tween 20) and Taq polymerase (Perkin-Elmer Inc., Wellesley MA) diluted to the manufacturers recommendations.

  • Because we created groups of samples diluted from the same individual RNA preparations, any group differences reflect differences in labeling and handling of the samples.

  • Isolated colonies were obtained at 100 ug/ml hygromycin and were passaged and diluted to extinction.

  • The solution was diluted with water to 100 ml (final concentration 20%) and stored at 4°C for not more than one week.


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