Words similar to diluted
Example sentences for: diluted
How can you use “diluted” in a sentence? Here are some example sentences to help you improve your vocabulary:
10 μl of the brush border membrane preparation was diluted in 90 μl of Laemmli buffer, boiled for 5 min and separated on a denaturing polyacrylamide gel (5 % stacking gel/10 % resolving gel) at 120 V over 2 hours [ 18 ] . The separated proteins were transferred onto nitrocellulose membrane using a Trans-blot instrument (Biorad, Hercules, CA) in glycine-methanol buffer following the manufacturer's suggestions.
In general, PCR reactions were performed in a volume of 50 μl with 50 picomoles of each primer, dNTP's (Amersham Pharmacia Biotech, Inc., Piscataway, NJ) at a final concentration of 0.5 mM, 1 μl of template, 1X PCR buffer (10X PCR buffer contained 700 mM Tris pH 8.8, 20 mM MgCl 2 , 1% Triton X-100, and 0.1% Tween 20) and Taq polymerase (Perkin-Elmer Inc., Wellesley MA) diluted to the manufacturers recommendations.
Because we created groups of samples diluted from the same individual RNA preparations, any group differences reflect differences in labeling and handling of the samples.
Isolated colonies were obtained at 100 ug/ml hygromycin and were passaged and diluted to extinction.
The solution was diluted with water to 100 ml (final concentration 20%) and stored at 4°C for not more than one week.