Example sentences for: diluted

How can you use “diluted” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • About 50 ml of the buffy coat was diluted 1:4 in RPMI and 25 ml was layered over 20 ml of Lymphoprep ™in a 50-ml conical centrifuge tube.

  • The appropriate number of cells was diluted into 50 μl sterile saline and then added to an equal volume of sterile Matrigel on ice.

  • The diluted samples (in duplicate) were then microcentrifuged for another 10 min and 25 μL samples taken from the top, middle and bottom of the fluid column in each tube, using a Hamilton syringe.

  • Primary antibody or non-immune rabbit serum (diluted 1:500) was applied overnight at 4°C.

  • Subsequently, washed slides were incubated with streptavidin-peroxidase conjugate for 15 minutes, washed again, and incubated 5 minutes in substrate-chromogen solution, rinsed with PBS, washed in distilled water, counterstained 2 minutes with Harris hematoxylin diluted 1:20, dehydrated, and mounted.


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