Words similar to dideoxy
Example sentences for: dideoxy
How can you use “dideoxy” in a sentence? Here are some example sentences to help you improve your vocabulary:
An in vitro assay using dideoxy terminated primer annealed with the template which allows the next correct dNTP to bind in the ternary complex without actual DNA synthesis has recently been reported [ 25 ] . Using this assay system, we have evaluated the ability of the insertion mutants to form the ternary complexes and the effect of DNA trap on such complexes.
The ternary complex (E-DNA-dNTP) formation was assessed by incubating the binary complexes of enzyme and dideoxy terminated template primer in the presence of next correct dNTP [ 25 ] . The binary complexes were formed by incubating 10-50 nM of the wild type enzyme or its mutant derivatives with 0.3 nM of 5'- 32P-labeled dideoxy terminated 33-mer/21-mer template-primer as described above.
Clones were analyzed by restriction digestion, and nucleotide sequencing was carried out on both strands by the dideoxy chain termination method.
All mutations were confirmed by dideoxy sequencing.
This is controlled by a mixture of dideoxy and deoxy nucleotide triphosphates, the ratio of which varies depending upon the assay design protocol obtained from the SpectroDESIGNER™ software (Sequenom).
Loading...