Example sentences for: dideoxy

How can you use “dideoxy” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • An in vitro assay using dideoxy terminated primer annealed with the template which allows the next correct dNTP to bind in the ternary complex without actual DNA synthesis has recently been reported [ 25 ] . Using this assay system, we have evaluated the ability of the insertion mutants to form the ternary complexes and the effect of DNA trap on such complexes.

  • The ternary complex (E-DNA-dNTP) formation was assessed by incubating the binary complexes of enzyme and dideoxy terminated template primer in the presence of next correct dNTP [ 25 ] . The binary complexes were formed by incubating 10-50 nM of the wild type enzyme or its mutant derivatives with 0.3 nM of 5'- 32P-labeled dideoxy terminated 33-mer/21-mer template-primer as described above.

  • Clones were analyzed by restriction digestion, and nucleotide sequencing was carried out on both strands by the dideoxy chain termination method.

  • All mutations were confirmed by dideoxy sequencing.

  • This is controlled by a mixture of dideoxy and deoxy nucleotide triphosphates, the ratio of which varies depending upon the assay design protocol obtained from the SpectroDESIGNER™ software (Sequenom).


How many words do you know? Try our free vocabulary size test!


Search

Search for example sentences

Loading Loading...
Quantcast