Example sentences for: dideoxy

How can you use “dideoxy” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • All DNA sequencing reactions were done using the dideoxy chain-termination protocol and T7 Sequenase v2.

  • In all, 142 templates were subjected to dideoxy chain termination sequencing.

  • Plasmids containing confirmed differentially expressed templates were re-cultured and plasmid DNA was extracted (Wizard Plus Mini-prep DNA Purification System, Promega Corporation, Madison, WI) and subjected to dideoxy chain termination sequencing (Applied Biosystems, Model 373A Automated Sequencer, Oklahoma State University Recombinant DNA/Protein Resource Facility).

  • An in vitro assay using dideoxy terminated primer annealed with the template which allows the next correct dNTP to bind in the ternary complex without actual DNA synthesis has recently been reported [ 25 ] . Using this assay system, we have evaluated the ability of the insertion mutants to form the ternary complexes and the effect of DNA trap on such complexes.

  • The ternary complex (E-DNA-dNTP) formation was assessed by incubating the binary complexes of enzyme and dideoxy terminated template primer in the presence of next correct dNTP [ 25 ] . The binary complexes were formed by incubating 10-50 nM of the wild type enzyme or its mutant derivatives with 0.3 nM of 5'- 32P-labeled dideoxy terminated 33-mer/21-mer template-primer as described above.

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