Example sentences for: diaminobenzidine

How can you use “diaminobenzidine” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After washing the sections 4 times in PBS, diaminobenzidine tetra-hydrochloride (DAB) was used to develop the color (dark brown).

  • After washing with PBS, the antibodies were detected using reagents in the Vectastain ABC Elite Kit (Vector Labs, Burlingame, USA); the sections were treated with biotinylated goat anti-rabbit IgG (at 1/200 dilution) for 30 min, rinsed with PBS, incubated with streptavidin-conjugated peroxidase for 30 min, washed with PBS, then treated with the chromogen 3,3'-diaminobenzidine for 5 min.

  • After washing with deionized water and PBS three times, species-specific secondary antibodies were added and the antibody complex was visualized by 3,3'-diaminobenzidine.

  • Antigen-antibody complexes were detected by standard diaminobenzidine technique (brown color).

  • Indirect immunoperoxidase staining was performed at 37°C for one hour using Vector Elite ABC kits (Vector, Burlingame, CA, USA) and diaminobenzidine (Kirkegaard and Perry, Gaitherburg, MD, USA) as a chromogen [ 11 12 ] . The polyclonal antibodies, goat antihuman Ang-1 and Ang-2, and rabbit antihuman Tie1 and Tie2, were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

How many words do you know? Try our free vocabulary size test!


Search for example sentences

Loading Loading...