Example sentences for: diaminobenzidine

How can you use “diaminobenzidine” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Indirect immunoperoxidase staining was performed at 37°C for one hour using Vector Elite ABC kits (Vector, Burlingame, CA, USA) and diaminobenzidine (Kirkegaard and Perry, Gaitherburg, MD, USA) as a chromogen [ 11 12 ] . The polyclonal antibodies, goat antihuman Ang-1 and Ang-2, and rabbit antihuman Tie1 and Tie2, were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

  • After washing the sections 4 times in PBS, diaminobenzidine tetra-hydrochloride (DAB) was used to develop the color (dark brown).

  • The sections were incubated in horseradish peroxidase-conjugated goat anti-rabbit and goat anti-mouse immunoglobulins for 30 min and developed with 3,3'-diaminobenzidine (DAB) Chromogen solution (DAKO).

  • Briefly, specimens were washed with phosphate buffered saline (PBS) and incubated 20 minutes with mouse-anti human Thy-1, clone F10-44-1 [ 33 34 ] (5 μg/ml in PBS) reacting with placental villous pericytes, extravillous fibroblasts, and decidual cells [ 15 ] . After extensive washing in PBS, specimens were incubated 20 minutes with swine anti-mouse IgG peroxidase conjugate (SwAM; SEVAC, Prague, Czech Republic) diluted 1:50 and preabsorbed with rat kidney homogenate [ 15 ] . Antigen-antibody complexes were detected by standard diaminobenzidine technique (brown color).

  • For labeling of immunoreactivity with the diaminobenzidine (DAB), the Vectastain Elite ABC-Kit (Vector Laboratories, Burlingame, CA) was used according to the suggested protocol.


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