Example sentences for: diaminobenzidine

How can you use “diaminobenzidine” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The color was developed with 17 mM 3',3'-diaminobenzidine, 40 mM hydrogen peroxide in 0.5 mM Tris-HCl buffer (pH 5.0).

  • After washing with deionized water and PBS three times, species-specific secondary antibodies were added and the antibody complex was visualized by 3,3'-diaminobenzidine.

  • AJCC, American Joint Committee on Cancer; Az value, area under ROC curves; DAB, Diaminobenzidine Hydrochloride; ROC curve, Receiver operating characteristic curve; S-100, S-100 protein.

  • Briefly, specimens were washed with phosphate buffered saline (PBS) and incubated 20 minutes with mouse-anti human Thy-1, clone F10-44-1 [ 33 34 ] (5 μg/ml in PBS) reacting with placental villous pericytes, extravillous fibroblasts, and decidual cells [ 15 ] . After extensive washing in PBS, specimens were incubated 20 minutes with swine anti-mouse IgG peroxidase conjugate (SwAM; SEVAC, Prague, Czech Republic) diluted 1:50 and preabsorbed with rat kidney homogenate [ 15 ] . Antigen-antibody complexes were detected by standard diaminobenzidine technique (brown color).

  • After being washed, cells were developed in 0.04% 3,3-diaminobenzidine tetrahydrochloride dihydrate (Sigma) with 0.02% H 2 O 2 in PBS for 15-30 min.


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