Example sentences for: diaminobenzidine

How can you use “diaminobenzidine” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After washing the sections 4 times in PBS, diaminobenzidine tetra-hydrochloride (DAB) was used to develop the color (dark brown).

  • After washing with PBS, the antibodies were detected using reagents in the Vectastain ABC Elite Kit (Vector Labs, Burlingame, USA); the sections were treated with biotinylated goat anti-rabbit IgG (at 1/200 dilution) for 30 min, rinsed with PBS, incubated with streptavidin-conjugated peroxidase for 30 min, washed with PBS, then treated with the chromogen 3,3'-diaminobenzidine for 5 min.

  • After washing with deionized water and PBS three times, species-specific secondary antibodies were added and the antibody complex was visualized by 3,3'-diaminobenzidine.

  • Antigen-antibody complexes were detected by standard diaminobenzidine technique (brown color).

  • Indirect immunoperoxidase staining was performed at 37°C for one hour using Vector Elite ABC kits (Vector, Burlingame, CA, USA) and diaminobenzidine (Kirkegaard and Perry, Gaitherburg, MD, USA) as a chromogen [ 11 12 ] . The polyclonal antibodies, goat antihuman Ang-1 and Ang-2, and rabbit antihuman Tie1 and Tie2, were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).


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