Words similar to deoxycholate
Example sentences for: deoxycholate
How can you use “deoxycholate” in a sentence? Here are some example sentences to help you improve your vocabulary:
5 mM mgCl2, 10 mM Hepes, pH 6.8, 0.1 mM dithiothreitol, 10 ug/ml cycloheximide, 0.5% Triton X-100, 0.5% deoxycholate) followed by 10 passages through a 22 gauge needle to lyse the cells.
0], 150 mM NaCl, 0.02% sodium azide, 0.1% SDS, 100 μg/ml phenylmethylsulfonyl fluoride, 1% NP-40, 0.5% sodium deoxycholate, and 10 μM sodium orthovanadate).
The supernatant fraction was diluted 1:1 with Buffer C (Buffer A containing 0.5% NP-40 and 0.5% deoxycholate, with no SDS) and loaded on to a Ni-NTA spin column (Qiagen).
The cell lysate was mixed with 0.25 ml of Buffer B (Buffer A containing 1% NP-40 and 1% deoxycholate) and the insoluble material was removed by centrifugation at 100,000 × g for 30 min.
15 M NaCl, 20 mM MgCl 2 , 0.025% SDS, 0.5% deoxycholate, 0.5% NP-40, 20 mM HEPES, pH 7.4) and the His 6 -tagged protein was eluted with Buffer D containing 0.25 M imidazole.