Words similar to deoxycholate
Example sentences for: deoxycholate
How can you use “deoxycholate” in a sentence? Here are some example sentences to help you improve your vocabulary:
Monolayers were then washed with cold PBS, pH 7.5, and solubilized in 1.0 ml of cell extraction buffer (50 mM HEPES, pH 7.5, 1% Triton X-100, 0.1% SDS, 0.25% deoxycholate, 150 mM sodium chloride, 1 mM EDTA, and protease inhibitors.
5 mM mgCl2, 10 mM Hepes, pH 6.8, 0.1 mM dithiothreitol, 10 ug/ml cycloheximide, 0.5% Triton X-100, 0.5% deoxycholate) followed by 10 passages through a 22 gauge needle to lyse the cells.
Cell lysates were obtained by rinsing 12-day old neuronal cell cultures with ice-cold phosphate-buffered saline prior to trituration in ice-cold lysis buffer (PBS supplemented with 1% Igepal, 0.5% sodium deoxycholate, 0.1% SDS, 100 μg/ml PMSF and 300 μg/ml aprotinin).
5%), sodium deoxycholate (0.
Bacteriologic methods were used as previously described [ 21 22 ] . Briefly, blood samples were centrifuged 15 min at 2,000 g and leukocytes recovered by standard methods with Ficoll-Hypaque (Pharmacia Fine Chemicals, Piscataway, NJ) and then mixed with a lysing solution that contained 0.45% sodium deoxycholate.
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