Example sentences for: deoxycholate

How can you use “deoxycholate” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The samples were washed two times with buffer-1, two times with buffer-2 (50 mM Tris-HCl, pH 7.5, 500 mM NaCl, 0.1% Nonidet P40 and 0.05% sodium deoxycholate) and one time with buffer-3 (50 mM Tris-HCl, pH 7.5, 0.1% Nonidet P40 and 0.05% sodium deoxycholate).

  • Cell lysates were obtained by rinsing 12-day old neuronal cell cultures with ice-cold phosphate-buffered saline prior to trituration in ice-cold lysis buffer (PBS supplemented with 1% Igepal, 0.5% sodium deoxycholate, 0.1% SDS, 100 μg/ml PMSF and 300 μg/ml aprotinin).

  • Total RNA from CHO.asbt and BN cells was prepared and analyzed by RNA blotting as previously described [ 17 ] . Cell lysates were prepared by lysing cells in 50 mM Tris-HCl (pH 7.4), 1% NP-40%, 0.25% sodium deoxycholate; 150 mM NaCl, 1 mM EGTA, 1 mM Na 3 VO 4 , 1 mM sodium fluoride, containing a protease inhibitor cocktail (Sigma-Aldrich Canada Ltd.).

  • 15 M NaCl, 20 mM MgCl 2 , 0.025% SDS, 0.5% deoxycholate, 0.5% NP-40, 20 mM HEPES, pH 7.4) and the His 6 -tagged protein was eluted with Buffer D containing 0.25 M imidazole.

  • Whole cell lysates were made from GC monolayers by standard techniques using a lysis buffer consisting of PBS with 1% Nonidet P40, 0.5% sodium deoxycholate, 0.1% SDS and protease inhibitor cocktail (1:100, Sigma P8340).

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