Words similar to deoxycholate

Example sentences for: deoxycholate

How can you use “deoxycholate” in a sentence? Here are some example sentences to help you improve your vocabulary:

15 M NaCl, 20 mM MgCl 2 , 0.025% SDS, 0.5% deoxycholate, 0.5% NP-40, 20 mM HEPES, pH 7.4) and the His 6 -tagged protein was eluted with Buffer D containing 0.25 M imidazole.
The SPM proteins were first incubated with 50 μl of protein G-agarose in buffer-1 (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1% Nonidet P40, 0.5% sodium deoxycholate, 2 μg/ml aprotinin, 5 mM EGTA, 4 mM Pefabloc SC, 1 μM pepstatin and 10 μg/ml leupeptin) at 4°C for 3 hours on a rocking platform to reduce background caused by non-specific absorption of irrelevant cellular proteins.
Total RNA from CHO.asbt and BN cells was prepared and analyzed by RNA blotting as previously described [ 17 ] . Cell lysates were prepared by lysing cells in 50 mM Tris-HCl (pH 7.4), 1% NP-40%, 0.25% sodium deoxycholate; 150 mM NaCl, 1 mM EGTA, 1 mM Na 3 VO 4 , 1 mM sodium fluoride, containing a protease inhibitor cocktail (Sigma-Aldrich Canada Ltd.).
5 mM mgCl2, 10 mM Hepes, pH 6.8, 0.1 mM dithiothreitol, 10 ug/ml cycloheximide, 0.5% Triton X-100, 0.5% deoxycholate) followed by 10 passages through a 22 gauge needle to lyse the cells.
The supernatant fraction was diluted 1:1 with Buffer C (Buffer A containing 0.5% NP-40 and 0.5% deoxycholate, with no SDS) and loaded on to a Ni-NTA spin column (Qiagen).