Words similar to deoxycholate
Example sentences for: deoxycholate
How can you use “deoxycholate” in a sentence? Here are some example sentences to help you improve your vocabulary:
137 M NaCl, 2% Nonidet P-40, 10% SDS, 1% sodium deoxycholate, 20 mM Tris, pH 8.0, 2 mM PMSF, 10 μl/ml protease inhibitor cocktail), which solubilizes cytoskeletal and nuclear matrices.
The samples were washed two times with buffer-1, two times with buffer-2 (50 mM Tris-HCl, pH 7.5, 500 mM NaCl, 0.1% Nonidet P40 and 0.05% sodium deoxycholate) and one time with buffer-3 (50 mM Tris-HCl, pH 7.5, 0.1% Nonidet P40 and 0.05% sodium deoxycholate).
The supernatant fraction was diluted 1:1 with Buffer C (Buffer A containing 0.5% NP-40 and 0.5% deoxycholate, with no SDS) and loaded on to a Ni-NTA spin column (Qiagen).
, IL) as described previously [ 50 81 ] . To determine the detergent solubility of the Rab24(D123I) mutant, transfected cells from a 10 cm culture dish were homogenized in 0.2 ml of 100 mM Tris-HCl, pH 7.4, containing 0.5% Nonidet P-40, 0.5% sodium deoxycholate, 0.1% SDS, and 2 mM EDTA.
The SPM proteins were first incubated with 50 μl of protein G-agarose in buffer-1 (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1% Nonidet P40, 0.5% sodium deoxycholate, 2 μg/ml aprotinin, 5 mM EGTA, 4 mM Pefabloc SC, 1 μM pepstatin and 10 μg/ml leupeptin) at 4°C for 3 hours on a rocking platform to reduce background caused by non-specific absorption of irrelevant cellular proteins.