Example sentences for: denhardt

How can you use “denhardt” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Nytran membranes with immobilized cDNAs for the BlueScript vector, human SP-A and human α-actin were prehybridized in 1 ml of buffer (50% formamide, 5 X SSC, 5 X Denhardt's solution, 100 μg/ml denatured herring sperm, 0.1% SDS) for 4 hours at 45°C and then hybridized to the labeled RNAs (6 × 10 6cpm in the presence of 500 units of RNasin) in duplicate for an additional 60 hours.

  • Hybridization was carried out with the 35S-labeled RNA probe (4-6 × 10 6cpm/ml) in a solution containing 50% (vol/vol) deionized formamide, 0.3 M NaCl, 10 mM Tris (pH 8.2), 1 mM EDTA, 0.05% yeast tRNA, 10 mM dithiothreitol, 1 × Denhardt's solution and 10% dextran sulfate.

  • The rat VEGF probe is a 0.9 kb cDNA Pst I/Sma I insert cloned into a pBluescript II KS+ vector [ 33 ] . Prehybridization and hybridization were performed in 50% formamide, 5×SSC (20×SSC is 0.3 M sodium chloride, 0.3 < sodium citrate), 10× Denhardt's solution (50× Denhardt's solution is 2% Ficoll, 2% polyvinyl pyrrolidone, 2% Bovine Serum Albumin Factor V), 50 mM sodium phophate (pH 7.0), 1% sodium dodecyl sulfate (SDS), and 250 μg/ml salmon sperm DNA at 42°C.

  • A rabbit genomic library in Lamda DASH ®II vector (Stratagene) was screened with [ 32P]-labeled full-length human EP 2 (gift from Dr. D. Woodward, Allergan Pharmaceuticals) [ 20 ] . The filters were fixed by ultraviolet light exposure (Stratalinker, Stratagene) and hybridized 18 hours at 56°C in a solution containing 6X SSC, 5X Denhardt's, 0.1% SDS, 150 μg/ml denatured herring sperm DNA and 3 × 10 6cpm/ml labeled probe.

  • The membrane was prehybridized for 2 hours at 42°C in 10 ml of hybridization buffer (final concentrations: 50% formamide, 5X Denhardt's solution, 0.1% SDS, 5X SSPE, 150 μg/ml denatured herring sperm DNA), and hybridized with the denatured probe overnight in 5 ml of hybridization buffer at 42°C.


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