Words similar to deneddylation
Example sentences for: deneddylation
How can you use “deneddylation” in a sentence? Here are some example sentences to help you improve your vocabulary:
So far, we were unable to obtain deneddylation of Pcu3p-13Myc in vitro by partially purified CSN preparations active in deneddylation of Pcu1p-13Myc (data not shown).
Our study also provides some insight into the role of deneddylation in cullin function, as we were able to create a situation in which Pcu1p and Pcu3p are fully neddylated in vivo (ref [ 10] and data presented here).
COP10, which has been shown to interact with AtCOP1 in yeast two-hybrid experiment, encodes a ubiquitin conjugating enzyme (E2) variant [ 44 ] . The COP9 signalosome, an eight-subunit complex homologous to the lid subcomplex of the 26 S proteasome [ 45 ] , directly associates with SCF E3 complex and promotes deneddylation of the cullin subunit of SCF in both plant and animal cells [ 46 47 48 49 ] . The exact relationship between COP1 and the COP9 signalosome remains obscure; it is known, however, that in Arabidopsis the COP9 signalosome is required for COP1 to accumulate in the nucleus in the dark [ 29 ] . Although COP1 most likely plays a role in regulating protein degradation, attempts in reconstituting ubiquitination of HY5 by AtCOP1 have been unsuccessful.
This mutant accumulates Pcu1p exclusively in a form carrying the stimulatory Nedd8p modification, due to a defect in COP9/signalosome-mediated cullin deneddylation [ 24 25 ] . Replacing wild-type ubiquitin by a mutant lacking all lysine residues prevented the formation of high molecular weight products (Fig.
The results presented here eliminate the attractive hypothesis that the cysteine box in Csn5p, which is conserved in numerous deubiquitylating enzymes [ 23], harbors a critical activity for deneddylation (Fig.
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