Words similar to degenerate
Example sentences for: degenerate
How can you use “degenerate” in a sentence? Here are some example sentences to help you improve your vocabulary:
R-PCR samples were generated from 50 ng restricted DNA using the Round A/ Round B protocol described in [ 9 12 ] . Briefly, Sequenase (Amersham) was used for two cycles of synthesis using a degenerate primer (5'-GTTTCCCAGTCACGATCNNNNNNNNN-3' ('round A').
Other highly conserved regions include TM1, TM2 and TM7; the last two domains are routinely used in the design of family-specific degenerate oligonucleotide primers for PCR amplification of ORs.
Although previous results of protein titration experiments suggested that KRC/ZAS-C binds DNA in a cooperative manner for a given oligonucleotide, the presence of multiple binding sites might not be favored over a single site in our site selection assay which used a degenerate pool of oligonucleotides and limited rounds of amplification.
We compared our IVT protocol to a commonly used PCR protocol that uses a partially degenerate primer to amplify genomic DNA in a sequence independent manner (R-PCR).
The IOP increase in the dark is not dependent on functional rod and cone photoreceptors since these cells degenerate in SWR/J mice due to homozygosity for the Pde6b rd 1mutation.