Words similar to degenerate

Example sentences for: degenerate

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To amplify gene-specific STSs, D. erecta, D. pseudoobscura, D. willistoni and D. littoralis genomic DNA was isolated from adult flies and used as template for PCR with degenerate primers.
Within the amino terminal sequences, yeast and other GCN2 proteins have a degenerate kinase domain of unknown function [ 31 ] . There is a 63 amino acid long sequence in IfkA that has 38% identity to the V and VI subdomains of the IfkA/B kinase domain, indicating the presence of an amino terminal pseudokinase domain in the Dictyostelium protein (fig.
The oligonucleotides in the degenerate pool were composed of twenty-five random nucleotides (25-mer) in the middle flanked by a specific sequence BSS1 at one end and the complementary sequence of BSS2 at the other end.
This strict requirement omitted binding sites that may have retained their function, despite mutations in degenerate positions that may have little impact on transcription factor binding.
Although previous results of protein titration experiments suggested that KRC/ZAS-C binds DNA in a cooperative manner for a given oligonucleotide, the presence of multiple binding sites might not be favored over a single site in our site selection assay which used a degenerate pool of oligonucleotides and limited rounds of amplification.