Words similar to d-akap

Example sentences for: d-akap

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Because D-AKAP1 was shown to associate with mitochondria [ 28 ] , double-labeling with markers for this organelle, either anti-lipoic acid or anti-cytochrome c, was used to examine whether RIα or D-AKAP1 are associated with mitochondria in the NMJ.
In our previous immunofluorescence work examining D-AKAP2 subcellular localization, the protein displayed a diffuse background in addition to mitochondrial staining [ 29 ] . It was thought that the combination of specific and diffuse labeling indicated the presence of more than one pool of D-AKAP2 inside the cells examined.
In contrast, the RIα labeling was invariably almost completely abolished when counterstained with bungarotoxin and anti-D-AKAP1 in multiple experiments (data not shown).
This observation is supported by previous work that demonstrated RIα codistribution with nAChR [ 19 ] and D-AKAP1 [ 17 ] suggesting that this AKAP is the anchoring protein recruiting RIα to this membrane.
A band for RIIβ was detected in contrast to a report of no RIIβ detection in soleus or extensor digitorum longus muscles [ 18 ] , which might be explained by the presence of nerve fibers or adipocytes wherein RIIβ is prevalent [ 27 ] . The blot for the affinity-purified D-AKAP1 antibody is shown because the protein-A purified antibody displayed additional, unexplained bands; however, no difference in fluorescence labeling was observed when using antibodies purified either way.