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Example sentences for: ctap
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Although CTAP is only a partial representation of the CAP molecule, the docking simulations agree with the experimental results.
The interacting region is in the RGPGRAF principal neutralizing domain (PND) of the V3 loop [ 52 ] . Both R311 and R315 residues are also in close proximity to two acid groups of the CTAP phthalic acid moiety and may take part in electrostatic interactions.
CAP, cellulose acetate phthalate; STD, sexually transmitted disease; ELISA, enzyme-linked immunosorbent assay; mAbs, monoclonal antibodies; FBS, fetal bovine serum; PEG 6000, polyethylene glycol 6000; HIV-1, human immunodeficiency virus type 1; BSA, bovine serum albumin; PBS, phosphate buffered saline; Ch-PBS, chicken serum (10%) in PBS; PBS-BG, 1% BSA/1% gelatin in PBS; HRP, horseradish peroxidase; sCD4, soluble CD4; TS, 0.14 M NaCl, 0.01 M Tris, pH 7.0; CTAP, cellotetraose acetate phthalate; pdb, Protein Data Bank; ED 50 , effective dose for 50 % inhibition.
CTAP interacts primarily through several hydrogen bonds with gp120 residues K362, N386, Q389, N392 and T394.
A cellulose chain consisting of one cellotetraose unit (composed of four 1,4-linked β-D-glucose units) was created in Quanta [ 22 ] and 50% of the hydroxyl groups at positions 2- and 3- were modified to acetyl ester and 25% of the hydroxyl groups at position 6 were modified to phthaloyl ester [ 1 23 ] . The acetylated and phthaloylated cellotetraose structure (CTAP) was minimized by the steepest descent method followed by the adopted basis Newton-Raphson (ABNR) method.