Words similar to cot-
Example sentences for: cot-
How can you use “cot-” in a sentence? Here are some example sentences to help you improve your vocabulary:
15 μg Human Cot-1 DNA was added to the probe before the first wash.
Resuspended probes were mixed with 4 μl of 5 × hybridization buffer (20 × SSX, 1%SDS), 1 μl human Cot-1 DNA (10 μg/μl), 1 μl polyA DNA (2 μg/μl) (Amersham) and 8 μl formamide (Sigma, St. Louis, MO), heated to 65°C for 2 min and then centrifuged briefly.
Filters were prehybridized in a solution containing 5 ml Microhyb (RGI), 5 μl mouse Cot-1 DNA and 5 μl poly(A) for 2 h at 42°C.
Cy3- and Cy5-labeled cDNA targets were then purified on QIAquick PCR purification columns, combined and concentrated by lyophilization, and hybridized to the microarray at 42°C for 16 h in hybridization solution containing 50% formamide, 5x SSC, 0.1% SDS, 20 μg mouse Cot-1 DNA and 10 μg poly(dA).
The probe was incubated with Cot-1 DNA (Life Technologies) at 70°C for 10 minutes before addition to the hybridization solution.
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