Words similar to cot-
Example sentences for: cot-
How can you use “cot-” in a sentence? Here are some example sentences to help you improve your vocabulary:
The probe was incubated with Cot-1 DNA (Life Technologies) at 70°C for 10 minutes before addition to the hybridization solution.
Resuspended probes were mixed with 4 μl of 5 × hybridization buffer (20 × SSX, 1%SDS), 1 μl human Cot-1 DNA (10 μg/μl), 1 μl polyA DNA (2 μg/μl) (Amersham) and 8 μl formamide (Sigma, St. Louis, MO), heated to 65°C for 2 min and then centrifuged briefly.
15 μg Human Cot-1 DNA was added to the probe before the first wash.
The labeled cDNA was mixed with 1.5 μl of 10 μg/μl Cot-1 DNA, 1.5 μl of 8 μg/μl poly d(A), 1.5 μl of 4 μg/μl yeast tRNA, 4.5 μl of 20x SSC and 0.75 μl of 10% SDS, heated at 99°C for 2 minutes, and then incubated at 45°C for 15 minutes.
Microarrays were pre-hybridized for 2 hours at 42°C with 5 ml of MicroHyb hybridisation solution (ResGen) plus 5 μg denatured Cot-1 DNA (ResGen) and 5 μg of poly dA.