Example sentences for: cot-

How can you use “cot-” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Resuspended probes were mixed with 4 μl of 5 × hybridization buffer (20 × SSX, 1%SDS), 1 μl human Cot-1 DNA (10 μg/μl), 1 μl polyA DNA (2 μg/μl) (Amersham) and 8 μl formamide (Sigma, St. Louis, MO), heated to 65°C for 2 min and then centrifuged briefly.

  • Filters were prehybridized in a solution containing 5 ml Microhyb (RGI), 5 μl mouse Cot-1 DNA and 5 μl poly(A) for 2 h at 42°C.

  • Microarrays were pre-hybridized for 2 hours at 42°C with 5 ml of MicroHyb hybridisation solution (ResGen) plus 5 μg denatured Cot-1 DNA (ResGen) and 5 μg of poly dA.

  • 15 μg Human Cot-1 DNA was added to the probe before the first wash.

  • Cy3- and Cy5-labeled cDNA targets were then purified on QIAquick PCR purification columns, combined and concentrated by lyophilization, and hybridized to the microarray at 42°C for 16 h in hybridization solution containing 50% formamide, 5x SSC, 0.1% SDS, 20 μg mouse Cot-1 DNA and 10 μg poly(dA).


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