Words similar to cot-
Example sentences for: cot-
How can you use “cot-” in a sentence? Here are some example sentences to help you improve your vocabulary:
15 μg Human Cot-1 DNA was added to the probe before the first wash.
The probe was incubated with Cot-1 DNA (Life Technologies) at 70°C for 10 minutes before addition to the hybridization solution.
The labeled cDNA was mixed with 1.5 μl of 10 μg/μl Cot-1 DNA, 1.5 μl of 8 μg/μl poly d(A), 1.5 μl of 4 μg/μl yeast tRNA, 4.5 μl of 20x SSC and 0.75 μl of 10% SDS, heated at 99°C for 2 minutes, and then incubated at 45°C for 15 minutes.
Filters were prehybridized in a solution containing 5 ml Microhyb (RGI), 5 μl mouse Cot-1 DNA and 5 μl poly(A) for 2 h at 42°C.
Cy3- and Cy5-labeled cDNA targets were then purified on QIAquick PCR purification columns, combined and concentrated by lyophilization, and hybridized to the microarray at 42°C for 16 h in hybridization solution containing 50% formamide, 5x SSC, 0.1% SDS, 20 μg mouse Cot-1 DNA and 10 μg poly(dA).