Words similar to cosmids
Example sentences for: cosmids
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The PCR product was used as a probe for colony blots to find cosmids in the E. coli (pYUB178::H37Rv) library carrying at least a portion of the cosmid integrated in the sur2 clone.
This represents about 20 M. tuberculosis genome-equivalents given that ~225 cosmids contain one genome-equivalent of M. tuberculosis [ 20 ] .
Because the recombinant clones contain integrated pYUB178::H37Rv cosmids, the following strategy was used to isolate cosmids corresponding to those in the sur2 and sur3 clones.
The pYUB178 plasmid and the λ phage library of pYUB178::H37Rv cosmids were generously provided by Dr. William Jacobs, Albert Einstein University, New York, NY [ 20 ] . The cosmid library contains 30-50 kb fragments of M. tuberculosis genomic DNA generated by partial Sau 3A digestion cloned into Bcl I-digested pYUB178.
Strains carrying different cosmids should exhibit a different pattern of hybridizing bands because of the presence of different M. tuberculosis H37Rv inserts.