Words similar to cosmid
Example sentences for: cosmid
How can you use “cosmid” in a sentence? Here are some example sentences to help you improve your vocabulary:
First, genomic DNA from the sur2 clone was digested with Pst I, and genomic DNA from the sur3 clone was digested with Bam HI to generate fragments of each integrated cosmid carrying oriE, aph, and a portion of the M. tuberculosis genomic DNA insert.
Cosmids carrying M. tuberculosis genes [ 20 ] were electroporated into M. smegmatis LR222 to create a library of M. smegmatis transformants, each of which carries a pYUB178::H37Rv cosmid integrated into its chromosome.
The pooled pYUB178::H37Rv cosmid DNAs were electroporated into M. smegmatis LR222, and kanamycin-resistant colonies were isolated.
The pYUB178 plasmid and the λ phage library of pYUB178::H37Rv cosmids were generously provided by Dr. William Jacobs, Albert Einstein University, New York, NY [ 20 ] . The cosmid library contains 30-50 kb fragments of M. tuberculosis genomic DNA generated by partial Sau 3A digestion cloned into Bcl I-digested pYUB178.
The PCR product was used as a probe for colony blots to find cosmids in the E. coli (pYUB178::H37Rv) library carrying at least a portion of the cosmid integrated in the sur2 clone.
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