Words similar to cosmid

Example sentences for: cosmid

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Two recent comparative sequence analyses clearly presented microsynteny of a 105 kb BAC DNA insert [ 10] and a 57 kb cosmid DNA insert [ 11] from tomato chromosomes 2 and 7, respectively, to their homeologous counterparts in the Arabidopsis genome.
A cosmid corresponding to the one in the sur3 clone was isolated from the E. coli (pYUB178::H37Rv) library by probing colony blots with the 4.5 kb Bam HI/ Eco Rl fragment of the M. tuberculosis genomic DNA insert in the plasmid recovered from the sur3 clone.
In experiments in which THP-1 cells were infected at a MOI of 50:1 (results in ~1 phagocytosed M. smegmatis bacterium per macrophage) with a mixture containing equal numbers of bacteria of the xylE -expressing control strain and a strain carrying the cosmid vector pYUB178, the ratio of recovered white colonies to yellow colonies was 1:1 at all time points (data not shown).
In this study, the enrichment process resulted in the isolation of two strains containing small, integrated plasmids rather than the expected 30-50 kb cosmids [ 20 ] . Small plasmids corresponding to the integrated ones were present in the original E. coli (pYUB178:H37Rv) cosmid library.
PCR, DNA sequence, and Southern blot data indicated that the recovered cosmid was the same as the cosmid integrated in the sur3 genome (data not shown).