Words similar to cosmid
Example sentences for: cosmid
How can you use “cosmid” in a sentence? Here are some example sentences to help you improve your vocabulary:
. Cosmid DNA from E. coli was prepared using the Qiagen Plasmid Maxi Kit (Qiagen, Valencia, Calif.) according to manufacturer's instructions for low-copy number plasmids.
Two recent comparative sequence analyses clearly presented microsynteny of a 105 kb BAC DNA insert [ 10] and a 57 kb cosmid DNA insert [ 11] from tomato chromosomes 2 and 7, respectively, to their homeologous counterparts in the Arabidopsis genome.
Cosmids carrying M. tuberculosis genes [ 20 ] were electroporated into M. smegmatis LR222 to create a library of M. smegmatis transformants, each of which carries a pYUB178::H37Rv cosmid integrated into its chromosome.
7 kb cosmid in the sur3 chromosome contains a 5.76 kb fragment of M. tuberculosis H37Rv genomic DNA (Figure 6) [ 23 ] . This region encodes eight potential ORFs designated Rv2233-Rv2240c as well as the tRNA for valine.
Portions of the E. coli (pYUB178::H37Rv) cosmid library were plated on LB agar containing 50 μg kanamycin/mL, and colony blots were performed with the ECL Direct Nucleic Acid Labeling & Detection System (Amersham) according to manufacturer's instructions.