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Example sentences for: coonrod

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This procedure is explained in detail in Coonrod et al, 1999 [ 8 ] . Briefly, after collection zona-free eggs were washed six times in BWW medium (Irvine Scientific, Santa Ana, CA) containing 100 μg/ml polyvinylalcohol (PVA, Sigma, St. Louis, MO), biotinylated with 2 mg/ml Sulfo-NHS biotin (Pierce, Rockford, IL) in BWW/PVA for 7 min at room temperature, and washed six times in BWW/PVA.
Two proteins in the unlabeled eggs bound streptavidin, corresponding to endogenous non-specific avidin binding proteins not included in the repertoire of surface-labeled proteins (data not shown, see figures in Coonrod et al.