Example sentences for: coomassie

How can you use “coomassie” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Protein concentration of the supernatant was determined with Coomassie Plus Protein Assay Reagent (Pierce).

  • The gels were then incubated for 24 hours at 37°C in 50 mM Tris-HCl buffer, pH 7.4, containing 5 mM CaCl 2 . A duplicate negative control gel was incubated as described above but in 50 mM Tris-HCl, pH 7.4 containing 10 mM EDTA instead of CaCl 2 . The gels were fixed for 1 hour in 40% methanol /7% acetic acid and then stained in Coomassie Blue Solution (Sigma), followed by destaining in 10% methanol, 7% acetic acid.

  • Each histone was resolved into multiple bands and were visualized in Coomassie blue gel.

  • The gel was stained with coomassie (BioRad, Hercules, CA) for 30 min., washed and abundant protein spots (as determined by intensity of staining) were cored and submitted for microsequencing by TMS.

  • Samples were then subjected to centrifugation to sediment myosin II filaments, and the proportion of myosin II in the pellet in each sample was quantified using SDS-PAGE and Coomassie blue staining as a measure of filament assembly (Figure 3A).


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