Words similar to coomassie

Example sentences for: coomassie

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To assemble Net1N beads with Cdc14 for six reactions (Figure 4A, detection method: Coomassie Blue staining), 105 μl of anti-T7 beads (Novagen) were incubated with 24 μg of His6-T7-Net1N in 100 μl of HEBD for 1 hr, washed four times with HBS + 0.2% n-octylglucoside + 2 mM DTT, and incubated with 6 μg of GST-Cdc14 in 100 μl of HEBD for 1 hr.
Staining the gel with Coomassie Brilliant Blue for each experiment assessed equal loading control.
All procedures were done at 4°C and the protein concentrations of samples were assayed using the Coomassie Blue method.
Protein concentration of the supernatant was determined with Coomassie Plus Protein Assay Reagent (Pierce).
Proteins were electroeluted from the non-denaturing gel as describe here [ 24 ] . Gels were fixed with fixing solution (50% methanol, 10% acetic acid) for 15 minutes, followed by staining in Coomassie staining solution (20% acetic acid, 0.025% Coomassie blue G-250) for one hour, followed by destaining in destaining solution (5% methanol, 10% acetic acid) [ 42 ] .