Words similar to cocl
Example sentences for: cocl
How can you use “cocl” in a sentence? Here are some example sentences to help you improve your vocabulary:
2 ; 0.06 mM 2-Mercaptoethanesulfonic Acid; 0.05 mg/mL BSA; 1 mM CoCl 2 ; 0.008 mM TdT dNTP mix; 300 U/mL TdT) and incubated for 60 minutes in a humidified chamber (37°C).
Plants ( Nicotiana glauca ) were grown in the greenhouse with supplemental illumination and fertilized as needed with a nutrient solution called CNS containing (2 mM CaCl 2 .2H 2 O, 0.5 mM MgSO 4 .7H 2 O, 2 mM KCl, 0.4 mM KH 2 PO 4 , 2.5 mM NH 4 NO 3 , 0.065 mM FeSO 4 .7H 2 O, 2.3 μM H 3 BO 3 , 0.9 μM MnSO 4 .H 2 O, 0.6 μM ZnSO 4 .7H 2 O, 0.1 μM NaMoO 4 .2H 2 O, 0.11 μM NiCl 2 .6H 2 O, 0.01 μM CoCl 2 .6H 2 O, 0.15 μM CuSO 4 .5H 2 O).
For polyadenylate tailing at the 3' end of the cDNA, 5 μl tailing buffer (200 mmol/l potassium cacodylate [pH 7.2], 4 mmol/l CoCl 2 , 0.4 mmol/l DTT), 2 mmol/l dATP (Roche, Indianapolis, IN, USA), and 10 U terminal transferase (Roche) were added, and incubated at 37°C for 20 min, followed by heat inactivation at 65°C for 10 min.
Up to 50 ng of CIP-treated template DNA were incubated for 20 minutes at 37°C in a 10 μL solution containing 20 U TdT (NEB), 0.2 M potassium cacodylate, 25 mM Tris-HCl pH 6.6, 0.25 mg/ml BSA, 0.75 mM CoCl 2 , 4.6 μM dTTP and 0.4 μM ddCTP.
After washing twice with 1.0 ml PBS, the cells were resuspended in 50 μl of TdT buffer containing 0.2 M potassium cacodylate, 2.5 mM Tris-HCL (pH 6.6), 2.5 mM CoCl 2 , 0.25 mg/ml bovine serum albumin, 5.0 units of terminal deoxynucleotidyl transferase and 0.5 nM biotinylated deoxyuridine triphosphate (b-UTP) and incubated at 37°C for 30 minutes.
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