Words similar to coating
Example sentences for: coating
How can you use “coating” in a sentence? Here are some example sentences to help you improve your vocabulary:
For coating with antibodies, wells were first coated with protein A/G (1 μg/well) in 0.1 M Tris buffer, pH 8.8 for 2 h at 20°C, followed by mAb NC-1 or normal mouse IgG (= control wells) {1 μg/well; diluted in phosphate buffered saline (PBS)} for 1 h at 20°C.
Cell growth, media, collagen coating and embedding in rafts
However, this coating would likely be only a few angstroms thick (ferrate was of very high purity at > 99% pure) and probably was insignificant compared to the overall amount of ferrate present.
Briefly, 6-well tissue culture plates were made non-adhesive with a coating of 1% agarose (Sea-Kem LE, FMC BioProducts, Rockland, MD).
For coating with antibodies, wells were first coated with protein A (1 μg/well) in 0.1 M Tris buffer, pH 8.8 for 2 h at 20°C followed by either mAbs (1 μg/well) or rabbit antisera diluted 1:100 in phosphate buffered saline (PBS) for 1 h at 20°C.