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Example sentences for: coated

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For cytokines assays, 1 × 10 6transduced cells were incubated on anti-CD3 coated plates for 72 h at 37°C/5% CO 2 . Culture supernatants were then harvested and an ELISA assay was performed to measure the production of IL-4, IL-10 and IFN-γ as previously described [ 30 ] . To assay proliferation, the transduced cells were plated in duplicate in microtiter plates (5 × 10 5/well) at a ratio of 1:5 with irradiated BALB/c splenocytes and the indicated doses of OVA peptide at 37°C/5% CO 2 . After 72 h, 50 μCi/ml [ 3H]thymidine (Amersham, Piscataway, NJ) was added to the well.
GFP-Arp3 cells were split onto 25 mm PLL coated coverslips 4-6 hrs before imaging.
The sandwich ELISA for the gp41 six-helix bundle was performed as described [ 21 ] . Treated and control virus preparations were incubated with lysis buffer for 30 min at 20°C and then added to wells coated with rabbit polyclonal antibodies to the gp41 core.
In the study reported here, we systematically compared the performance of unmodified 70-mer oligonucleotides to traditional PCR amplicons, both probe types printed and UV cross-linked onto glass slides coated with primary amine groups.
Implantation of trypsin coated beads at stage 17 had no effect on either the timing of nerve development (axonal or glial) or the biochemical differentiation of the axons.