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Example sentences for: coated

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Cells were either left in suspension (for suspended and PDGF stimulated samples) or were seeded onto the coated wells (1 ml per well) and allowed to adhere for 30 min at 37°C, or for the times indicated in the figure legend (Fig.
For cytokines assays, 1 × 10 6transduced cells were incubated on anti-CD3 coated plates for 72 h at 37°C/5% CO 2 . Culture supernatants were then harvested and an ELISA assay was performed to measure the production of IL-4, IL-10 and IFN-γ as previously described [ 30 ] . To assay proliferation, the transduced cells were plated in duplicate in microtiter plates (5 × 10 5/well) at a ratio of 1:5 with irradiated BALB/c splenocytes and the indicated doses of OVA peptide at 37°C/5% CO 2 . After 72 h, 50 μCi/ml [ 3H]thymidine (Amersham, Piscataway, NJ) was added to the well.
A second type of vesicle, coated by the COPI coat-protein complex, is part of a retrograde pathway that buds off the Golgi membrane and returns proteins that are not targeted for secretion back to the endoplasm [ 3, 4].
Enzyme was coated onto the plates at 4°C overnight.
For imaging experiments the neurons were transferred to 4-chamber cover glass slides that had been previously coated with poly-L-lysine followed by collagen.