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Example sentences for: cncs

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To test the hypothesis that CNCS clusters can predict the location of cis -regulatory elements in the Drosophila genome, we carried out P -element-mediated reporter gene analysis of genomic sequences corresponding to a CNCS cluster in the fourth intron of ap . This CNCS cluster is apparent in pairwise comparisons between D. melanogaster and D. pseudoobscura as well as between
Changes in the median CNCS length reflect changes in the overall distribution of CNCS lengths in pairwise comparisons between D. melanogaster and either D. erecta, D. pseudoobscura, D. willistoni , or D. littoralis (Figure 5).
Clustering of CNCSs and conservation of CNCS spacing are not expected under a mutational cold-spot hypothesis, and suggest that the spacer intervals between CNCSs, and therefore CNCSs themselves, are under functional constraint.
As shown in Figure 8, the D. melanogaster genomic sequences corresponding to the CNCS cluster in the ap intron 4 drives reporter gene expression in the Drosophila embryo in a specific pattern that recapitulates native ap expression in the developing brain.
The stability of this distribution at more extreme evolutionary distances is apparently insensitive to changes in the proportion of non-coding DNA that is conserved (compare D. willistoni and D. littoralis ). Shown for comparison is the distribution of CNCS lengths between D. melanogaster and D. virilis from [ 44], as well as a reanalysis of this data using the current methods.