Words similar to clonally
Example sentences for: clonally
How can you use “clonally” in a sentence? Here are some example sentences to help you improve your vocabulary:
From four original fibroblast samples, we generated clonally derived cell strains that expressed only the mutant MeCP2 or only the wild-type MECP2 allele, but not both.
The use of clonally derived fibroblasts eliminated the mosaic X inactivation seen in tissues from most females with Rett syndrome.
The use of lymphoblastoid cell lines, while not all clonally derived, expanded the scope of genes to be assayed beyond those expressed in skin fibroblasts.
Cortical astrocytes were harvested from neonatal C57BL/6 mice and infected with a replication-defective retrovirus encoding Tag and neomycin resistance as previously described [ 13 ] . The transferred viruses were SV40-6 [ 5 ] , in which wild type Tag is expressed from the M-MuLV LTR promoter; SV(X)T K1, which is constructed the same as SV40-6 except that the Tag is mutant [ 38 ] and defective for binding the retinoblastoma protein family [ 39 ] ; Linker-tkT, in which the cDNA for Tag is expressed from the herpes simplex 1 tk promoter [ 11 ] . The differentiation and transformation phenotypes of the cell lines utilizing the M-MuLV LTR promoter have been described [ 6 13 ] . The Linker-tkT lines (tkT) were either isolated as clones after G418 selection (as previously described) [ 6 ] or frozen immediately after selection (pooled clones) and clonally selected at a later time.
In order to confirm that these clonally expanded B cells were receiving ongoing antigenic stimulation and not limited solely to the memory compartment, we employed the cDNA-based assay to distinguish clonal expansions of activated B cells from resting (memory) cells.
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