Words similar to circularized

Example sentences for: circularized

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Similarly, OCP ligation/ERCA can be used to accurately genotype PCR products in a biallelic format, where the probes for both alleles are present in a single reaction [ 3 ] . The OCP ligation/ERCA method is based on ligation dependent circularization of allele specific DNA probes followed by exponential rolling circle amplification of the circularized probes [ 4 5 ] . ERCA probes have also been variously described as 'padlock probes' [ 6 7 ] or 'circularizable probes' [ 4 ] . Although OCP ligation/ERCA can be used to genotype PCR products, it also provides a means for directly genotyping genomic DNA without requiring an initial amplification of the genomic DNA locus.
An electrophoretic mobility shift for circularized OCP allowed quantification of ligation kinetics.
Exponential amplification of circularized OCP requires primers P1 and P2 (see Figure 2).
For each time point, the number of counts in unreacted intron, spliced intron, and circularized intron were determined.
In reactions where the OCP matches the SNP, an estimated 100-30,000 OCPs are circularized by ligation depending on the amount of input DNA target.