Example sentences for: circularized

How can you use “circularized” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Exponential amplification of circularized OCP requires primers P1 and P2 (see Figure 2).

  • The reaction was heated to 95°C for 10 seconds, and cooled to 63-68°C for 5-20 minutes, during which time OCP annealed to genomic target and was circularized by ligase.

  • With both mutant and normal OCP in the reaction, either one or the other or both OCPs were circularized, depending on the genotype.

  • Similarly, OCP ligation/ERCA can be used to accurately genotype PCR products in a biallelic format, where the probes for both alleles are present in a single reaction [ 3 ] . The OCP ligation/ERCA method is based on ligation dependent circularization of allele specific DNA probes followed by exponential rolling circle amplification of the circularized probes [ 4 5 ] . ERCA probes have also been variously described as 'padlock probes' [ 6 7 ] or 'circularizable probes' [ 4 ] . Although OCP ligation/ERCA can be used to genotype PCR products, it also provides a means for directly genotyping genomic DNA without requiring an initial amplification of the genomic DNA locus.

  • For each time point, the number of counts in unreacted intron, spliced intron, and circularized intron were determined.


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