Words similar to circularization
Example sentences for: circularization
How can you use “circularization” in a sentence? Here are some example sentences to help you improve your vocabulary:
The ERF protein of phage P22 is involved in the circularization of the linear dsDNA phage genome upon entry into the host cell [ 21 22 23 ] . Experimental studies have shown that, mutations in ERF are complemented by Redβ and that in vitro ERF adopts quaternary structures analogous to those of Redβ and RecT [ 14 17 24 25 ] . However, in the comprehensive analysis of the RecT/Redβ superfamily no statistically significant similarity could be detected between these proteins and the ERF proteins.
A mismatch between the OCP and the SNP prevents ligation and circularization.
Allele specificity of circularization is dependent upon two factors: 1) favorable hybridization of the matching OCP because of the correct base pair formed at the 3' end and also because of base stacking between the 3' and 5' annealed ends, and 2) specificity of the ligase for correctly base paired ends.
Upon OCP circularization, an isothermal exponential RCA (ERCA) reaction involving an exonuclease (-) DNA polymerase with strand-displacement activity and two primers rapidly amplifies the signal by as much as 10 12-fold, allowing for direct SNP genotyping from small quantities of DNA target [ 6 11 12 ] .
Similarly, OCP ligation/ERCA can be used to accurately genotype PCR products in a biallelic format, where the probes for both alleles are present in a single reaction [ 3 ] . The OCP ligation/ERCA method is based on ligation dependent circularization of allele specific DNA probes followed by exponential rolling circle amplification of the circularized probes [ 4 5 ] . ERCA probes have also been variously described as 'padlock probes' [ 6 7 ] or 'circularizable probes' [ 4 ] . Although OCP ligation/ERCA can be used to genotype PCR products, it also provides a means for directly genotyping genomic DNA without requiring an initial amplification of the genomic DNA locus.
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