Words similar to cholate

Example sentences for: cholate

How can you use “cholate” in a sentence? Here are some example sentences to help you improve your vocabulary:

Cells were washed once with ice-cold PBS and lysed in 1 ml of Extraction buffer (50 mM HEPES, pH 8, 50 mM NaCl, 10 μM β-mercaptoethanol, 1% Triton X-100, 1% sodium cholate, 1 mM PMSF, 2 μg/ml leupeptin, and 2 μg/ml aprotinin).
The cells were again collected by centrifugation (6000 rpm × 30 min), then lysed with 20 ml of 20% sodium cholate, pH 7.1 (30 min., 4°C).
5 g/L of Sigma Bile Salts, consisting of 50% sodium cholate, 50% sodium deoxycholate), which is typically used to identify enteric bacteria.
PR was obtained in 85% purity, assuming that values of ε 280 and ε 546 for pR are the same as for bR solubilized in DMPC/cholate/SDS mixtures at pH 8 (ε 280 = 7.85 × 10 4cm -1M -1and ε 551 = 4.8 × 10 4cm -1M -1) [ 5 ] . This assumption is actually expected to underestimate the purity of pR produced, by up to ~20%, since the pR we expressed has 10 tryptophan and 14 tyrosine residues, as compared to 8 tryptophans and 11 tyrosines in bR from H. salinarum.
After extracting 3× more with the same cholate solution, the pooled supernatants were diluted 10× with buffer A and centrifuged at 180,000 g for 45 min to collect the membrane pellet.