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Example sentences for: chimeras

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This region was identified using chimeras of α 1S and α 1C [ 16 ] . Since Csk53 is present in α 1S Δ1-700 and was detected by the II-III loop antibody which is directed against the center portion of Csk53 [ 34 ] , the participation of this domain in the EC cannot be ruled-out.
Although syk -deficient mice die perinatally, analysis of radiation chimeras reconstituted with fetal liver from syk -deficient mice has demonstrated a block in the transition from proB cells to preB cells, indicating that signal transduction through Syk is required for early B cell development [ 35 36 ] . Inactivation of the syk gene in the chicken DT40 B cell lymphoma leads to a loss in the activation of PLCγ2, the increase in intracellular free Ca 2+and the apoptotic response following engagement of the BCR.
Targeted ES cell clones were injected into C57Bl/6J blastocysts and chimeras obtained.
Cell lysis, Immunoprecipitation, SDS-PAGE, and Western blotting were performed exactly as described [ 48 ] . Tyrosine phosphorylation of chimeras and other substrates were determined following immunoprecipitation using the indicated antibodies and probing with RC20-HRP anti-phosphotyrosine antibodies.
These were expanded and used to produce germline chimeras.