Words similar to checkpoint
Example sentences for: checkpoint
How can you use “checkpoint” in a sentence? Here are some example sentences to help you improve your vocabulary:
In high-throughput applications such as routine diagnostic examinations, where a large number of hybridizations may be performed using a standard microarray-design, the ROC-plot area may be used as a 'hybridization quality checkpoint' to either accept or discard individual microarray hybridizations (for example 0.990 for array 1 and 2, Table 2).
The MATs are predicated upon the coordinated activities of the spindle checkpoint [ 16 17 ] , the anaphase-promoting complex (APC) or cyclosome [ 18 19 ] , the proteasome [ 20 21 ] , and the cohesion-complex proteins involved with chromosome cohesion and separation [ 22 23 24 ] . Alterations in the temporal sequence of these coordinated activities may potentially predispose cells to faulty chromosome segregation.
Indeed, p53 controls the low-level damage-dependent premeiotic checkpoint which facilitates DNA repair during spermatogenesis [ 25 ] . Gametogenesis is inherently error-prone [ 26 ] and subject to mutations [ 27 ] . Nevertheless, it is imperative to avoid genotoxic complications that could become integrated into the germline.
Rad26 is a regulatory subunit of the Rad3 checkpoint kinase [ 18 ] . To investigate the cellular distribution of Rad26, we used a one step PCR-based gene-modification method [ 41 ] to fuse GFP (S65T) to the C-terminus of the genomic copy of rad26 +. The Rad26-GFP fusion protein maintained proper checkpoint function following hydroxyurea (HU) and Bleomycin treatments (data not shown).
To activate the DNA damage checkpoint, Bleomycin was added to cultures (optical density of 0.3) at a concentration of 5 mU/ml for 3 hours [ 28 ] . Thiabendazole (Sigma) was prepared in DMSO as described previously [ 45 ] and added to cooled, YE5S agar prior to pouring plates.
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