Words similar to cell-matrix
Example sentences for: cell-matrix
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TSP-1 is a homotrimeric multidomain glycoprotein synthesized and secreted by numerous cell types, including platelets, vascular smooth muscle cells and keratinocytes [ 4 ] . Due to its interaction with a wide variety of proteins, TSP-1 has been implicated in a number of biological processes including coagulation, cell adhesion, the modulation of cell-cell and cell-matrix interactions, control of tumor growth and metastasis, and angiogenesis [ 10 ] . Inhibition of tumor cell proliferation has been achieved in vivo by systemic treatment with TSP-1 peptide mimetics [ 11 ] , but a correlation between primary tumor TSP-1 expression and poor prognosis has not been clearly established [ 12 ] . While there are reports that these molecules can originate from tumor cells [ 13 ] , the majority of reports have suggested that the major source of both TSP-1 and OPN is tumor-associated stroma [ 14 ] or infiltrating macrophages and lymphocytes [ 15 ] .
Because essentially all of these previous studies have analyzed PTEN function by introducing the cloned version of the gene back into PTEN-deficient cells, essentially nothing is known about the regulation of the endogenous PTEN gene in response to alterations of cell-matrix interactions.
In parallel, the expression of cyclin A, an essential component of certain cyclin-dependent kinases and absolutely required for the progression of cells through the cell cycle [ 38 39 ] , was down-regulated under these conditions (Figure 1), consistent with earlier observations [ 27 28 31 ] . Furthermore, expression of the CDK inhibitor p27 Kip1was strongly increased in suspension cells (Figure 1), similar to what has been observed in other cell types after the disruption of cell-matrix interactions [ 29 31 32 ] . Finally, as we have reported before [ 31 ] , the expression of cdk4, one of the catalytic subunits of CDKs, was not significantly altered in suspension culture cells and therefore could be used as a loading control for the Western blot analysis.
In light of the close correlation between the anchorage-independent phenotype and the tumorigenicity of transformed cells, it is important to fully understand the cellular mechanisms that are involved in cell growth arrest after the disruption of cell-matrix interactions.
Previously, studies of phenotypes in this laboratory have shown that SPARC-null mice develop osteopenia around 2.5 months of age [ 19 ] in bones where SPARC is normally the most abundant non-collagenous glycoprotein [ 3 ] . The second phenotype thus far uncovered is in the lens where SPARC is normally produced by lens epithelial cells [ 20 21 ] and is a component of the lens capsule [ 20 22 ] . SPARC-null mice show progressive cataract formation beginning approximately 1.5 months after birth [ 14 21 ] . Electron microscopy of the SPARC-null lens revealed abnormality at the lens cell-ECM (capsule) interface with an intrusion of cellular processes into the basement membrane of the lens capsule, whereas wild-type lens exhibited a precise border at the cell-matrix interface [ 23 ] . In the present study, we show for the first time that SPARC plays a role in wound repair in vivo and in vitro.
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