Words similar to cdnas
Example sentences for: cdnas
How can you use “cdnas” in a sentence? Here are some example sentences to help you improve your vocabulary:
Nytran membranes with immobilized cDNAs for the BlueScript vector, human SP-A and human α-actin were prehybridized in 1 ml of buffer (50% formamide, 5 X SSC, 5 X Denhardt's solution, 100 μg/ml denatured herring sperm, 0.1% SDS) for 4 hours at 45°C and then hybridized to the labeled RNAs (6 × 10 6cpm in the presence of 500 units of RNasin) in duplicate for an additional 60 hours.
An independent project to sequence complete Arabidopsis cDNAs is ongoing by the SPP consortium [ 28], using clones created by K. Shinozaki at RIKEN in Japan.
Primary cultures were prepared from hind limbs of day 18 embryos (E18) as described previously [ 23 ] . cDNAs of interest and a separate expression vector encoding the T-cell membrane antigen CD8 were subcloned into the mammalian expression vector pSG5 (Stratagene, CA) and were mixed and cotransfected with the polyamine LT-1 (Panvera, WI).
These arrays include 1176 mouse cDNAs, 9 housekeeping control cDNAs, and negative controls immobilized on a nylon membrane www.clontech.com.
The frequency of such introns in Drosophila is low; 32 are annotated in Release 3. There are several well supported examples of introns less than 45 bp, with at least two supporting cDNAs derived from the sequenced strain.