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Example sentences for: cdnas

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The template DNA used for making probes to detect expression of PR-1 , PR-5 and ROC1 (cyclophilin, used to normalize for RNA loading) were PR-1 and PR-5 cDNAs [ 14 ] and an Eco RI fragment of pCG22 [ 34 ] , respectively.
In brief, the Drosophila Gene Collection (DGC 1.0, kindly provided by the Berkeley Drosophila Genome Project) of 5,849 nonredundant cDNAs was amplified using universal primers essentially as described at [ 42].
The GAL4 fusion plasmids were obtained by subcloning the appropriate cDNAs into the pGaL4PolyII plasmid [ 24 ] , in-frame with the yeast GAL4 binding domain coding sequence.
The predicted protein sequences of the derived cDNAs were analyzed for the presence of functional domains using the conserved domain function of BLAST at NCBI as well as the Simple Modular Architecture Research Tool at http://smart.embl-heidelberg.de.
In summary, the present study provides evidence that the performance of unmodified 70-mer oligonucleotides is comparable to cDNAs printed on glass slides.