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Example sentences for: cdnas

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The PCR-Select cDNA Subtraction and PCR-Select Differential Screening Kits (Clontech Laboratories Inc.) were used to enrich for and identify differentially expressed genes in the driver and tester cDNAs [ 48 49 ] . Both forward- and reverse-subtraction were performed; untreated HUVEC cDNA served as driver and VEGF-treated HUVEC cDNA served as tester in the former, whereas untreated HUVEC cDNA served as tester and VEGF-treated HUVEC cDNA served as driver in the latter [ 48 49 ] . Target cDNA fragments were amplified using the Marathon™ cDNA Amplification Kit, subcloned into pCR ®2.
Rat cDNAs for Abcg5 and Abcg8 were identified previously by amplification of these sequences from a Sprague Dawley rat enterocyte cDNA library, using primers located in areas conserved between mouse and human (Genbank AF312714 and AF351785 respectively).
COP1 cDNAs.
The fragment lengths from single clone analyses that by chance contained cDNAs from the same gene reproduced very accurately.
The same RNA samples were used to probe Atlas mouse cDNA expression array (588 genes) and Stress/Tox array (149 genes) (Clontech Laboratories), 32P-labeled cDNAs were produced from 2 μg of each pooled RNA, using the reagents supplied with the membrane array kits.