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Example sentences for: capan-
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It should be noted that polony amplification of genomic DNA from strains with equal p53 and K- ras 2 copy numbers, such as CAPAN-1, yielded equivalent numbers of p53 and K- RAS 2 polonies (data not shown).
The pancreatic cell lines AsPc-1, CAPAN-1 and Panc-1 were purchased from the American Type Culture Collection (Manassas, VA) and were grown according to the manufacturer's instructions [ 28 29 30 ] . Genomic DNA was harvested from these cells using a Qiagen (Alameda, CA) Blood and Cell Culture DNA Midi Kit.
In addition to the cell line Panc-1, K- ras 2 mutations in the second position of codon 12 were also shown to be present in the cell lines AsPC-1 (G → A; data not shown) and CAPAN-1 (G → T; data not shown).
In contrast, in the TGFβ 1 -resistant AsPC-1 and Capan-1 cells, the interaction of mature TGFβ 1 with α V β 6 integrin resulted in an immobilization of high molecular weight proteins to the cytoskeleton without tyrosine phosphorylation (Figure 2).
The cell lines Capan-1 and AsPC-1 are not only resistant to TGFβ 1 because of their alterations in the SMAD pathway, but also because they cannot complete the alternate pathway, as demonstrated above.