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Example sentences for: capan-
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In contrast, in the TGFβ 1 -resistant AsPC-1 and Capan-1 cells, the interaction of mature TGFβ 1 with α V β 6 integrin resulted in an immobilization of high molecular weight proteins to the cytoskeleton without tyrosine phosphorylation (Figure 2).
In addition to the cell line Panc-1, K- ras 2 mutations in the second position of codon 12 were also shown to be present in the cell lines AsPC-1 (G → A; data not shown) and CAPAN-1 (G → T; data not shown).
The pancreatic cell lines AsPc-1, CAPAN-1 and Panc-1 were purchased from the American Type Culture Collection (Manassas, VA) and were grown according to the manufacturer's instructions [ 28 29 30 ] . Genomic DNA was harvested from these cells using a Qiagen (Alameda, CA) Blood and Cell Culture DNA Midi Kit.
The cell lines Capan-1 and AsPC-1 are not only resistant to TGFβ 1 because of their alterations in the SMAD pathway, but also because they cannot complete the alternate pathway, as demonstrated above.
It should be noted that polony amplification of genomic DNA from strains with equal p53 and K- ras 2 copy numbers, such as CAPAN-1, yielded equivalent numbers of p53 and K- RAS 2 polonies (data not shown).