Words similar to cap-gp

Example sentences for: cap-gp

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CAP-gp120 wells were prepared by coating first with HIV-1 IIIB gp120 under conditions described above for protein A/G, except that the pH was 8.0 instead of 8.8.
Quantitation of CAP-gp120 binding
Interestingly, CAP-gp120 binding also occurs in 1M NaCl, conditions under which binding of sulfated polysaccharides to gp120 is abolished [ 43 ] . This suggests that electrostatic interactions do not play an exclusive role in CAP-gp120 binding [ 51 ] .
25 to 8 μg/ml) of the mAb and of control mouse IgG, respectively (each at 16 μg/ml in PBS-BG) were added to CAP coated or CAP-gp120 coated wells for 1 h at 37°C.
CAP retained on top of the filter was quantitated as a complex with ruthenium red [ 21 ] . The retentate on the 100,000 M w cut-off filter was dissolved in 1 ml of 2 M guanidinium hydrochloride and CAP released from the CAP-gp120 complex and retained on the 50,000 M w cut-off filter was quantitated by the same method.