Words similar to cap-independent
Example sentences for: cap-independent
How can you use “cap-independent” in a sentence? Here are some example sentences to help you improve your vocabulary:
It was recently demonstrated that elements within a 217 nucleotide sequence from the 5'-UTR of the mouse p27 Kip1mRNA is able to mediate cap-independent translation of a reporter gene in NIH3T3 and D6P2T cells [ 28 ] . There is a high level of sequence homology between the mouse and human 5'-UTRs and the major transcriptional start site is also conserved [ 29 ] . Therefore it is likely the 5'-UTR of the human p27 Kip1message is also able to mediate cap-independent translation initiation.
One possibility is that binding of 4EBP-1 to eIF4E releases other initiation factors that are in limited supply but also required for cap-independent initiation.
It has been shown that expression of p27 Kip1is controlled at the translational level [ 3 26 29 ] and that the p27 Kip15'- UTR is able to mediate enhanced translation rates [ 31 ] . In addition, it was recently reported that the p27 Kip15'- UTR has an internal ribosome entry site that allows cap-independent initiation of translation [ 30 ] . However, none of these reports have utilized the full 5'- UTR that results from transcription initiation at the major start site.
This property is shared by the highly homologous mouse p27 Kip15'-UTR [ 28 ] . Coexpression of 4EBP-1-5A with the bicistronic construct containing the p27 Kip15'-UTR leads to an approximately two-fold increase in cap-independent expression.
The increase in p27 Kip1is mediated, at least in part, by enhanced synthesis of the protein and corresponds to activation of the p27 Kip1mRNA 5'-untranslated region (UTR) and its ability to mediate cap-independent initiation of translation.
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