Words similar to cap-independent
Example sentences for: cap-independent
How can you use “cap-independent” in a sentence? Here are some example sentences to help you improve your vocabulary:
Thus the full-length human p27 Kip15'-UTR is able to mediate cap-independent translation in a manner similar to that previously shown for the 217 nucleotide sequence of the mouse 5'-UTR [ 28 ] .
It has been shown that expression of p27 Kip1is controlled at the translational level [ 3 26 29 ] and that the p27 Kip15'- UTR is able to mediate enhanced translation rates [ 31 ] . In addition, it was recently reported that the p27 Kip15'- UTR has an internal ribosome entry site that allows cap-independent initiation of translation [ 30 ] . However, none of these reports have utilized the full 5'- UTR that results from transcription initiation at the major start site.
It was recently demonstrated that elements within a 217 nucleotide sequence from the 5'-UTR of the mouse p27 Kip1mRNA is able to mediate cap-independent translation of a reporter gene in NIH3T3 and D6P2T cells [ 28 ] . There is a high level of sequence homology between the mouse and human 5'-UTRs and the major transcriptional start site is also conserved [ 29 ] . Therefore it is likely the 5'-UTR of the human p27 Kip1message is also able to mediate cap-independent translation initiation.
The second cistron, encoding luciferase, is downstream of the CAT stop codon and therefore must be translated by a cap-independent mechanism mediated by sequence elements inserted between the two cistrons.
The increase in p27 Kip1is mediated, at least in part, by enhanced synthesis of the protein and corresponds to activation of the p27 Kip1mRNA 5'-untranslated region (UTR) and its ability to mediate cap-independent initiation of translation.
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