Words similar to cap-dependent
Example sentences for: cap-dependent
How can you use “cap-dependent” in a sentence? Here are some example sentences to help you improve your vocabulary:
IRES entirely contained within a translated ORF has been shown in the MMLV gag [ 47 ] , and host mRNA encoding p110 PITSLREand p58 PITSLRE [ 48 ] . Along these lines, cap-dependent translation may be cell cycle regulated, especially when cells are arrested at the G2 phase of the cell cycle, where the cap-dependent translation of most cellular host cell mRNAs is inhibited [ 49 50 51 ] .
To test this, a 472 nucleotide fragment, representing the full-length human p27 Kip15'-UTR derived from transcription initiation at the major start site, was inserted into the bicistronic expression vector pGL2CAT/Luc [ 30 ] . In this vector, the first cistron, encoding chloramphenicol acetyltransferase (CAT), is proximal to the mRNA's cap structure and therefore expected to be translated by the conventional cap-dependent translation mode.
Phosphorylation of 4EBP disrupts its ability to bind eIF4E, freeing eIF4E to bind eIF4G, which functions to recruit additional components of the initiation complex [ 7 8 ] . The net result is that phosphorylation of 4EBP enhances cap-dependent translation initiation.
(iii) Lack of interferon response: We provide direct experimental evidence that the 21-nt dsRNA does not trigger an IFN response, which is an advantage because an IFN response would have caused a general and non-specific inhibition of all cap-dependent translation [ 5 ] . (iv) Regulation any time: Although we have not tested this specifically, one can, in principle, transfect the virus-infected cell with dsRNA at any time point during infection.
Since, the TAR is located at the 5' end of all HIV-1 RNA transcripts and the presence of secondary structure at or near the 5' end of RNAs reduces the accessibility of the 5' cap to eIF4F, it is thought that this feature of HIV-1 mRNAs can inhibit their cap-dependent translation [ 44 45 46 ] . Therefore, a possible function of the HIV-1 gag IRES might be to serve as a mechanism to bypass the structural barriers to cap-dependent translation by recruiting ribosomes easily and directly to the gag ORFs.
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