Words similar to cap-dependent
Example sentences for: cap-dependent
How can you use “cap-dependent” in a sentence? Here are some example sentences to help you improve your vocabulary:
Expression of constitutively active 4EBP-1 should lead to a global decrease in cap-dependent translation which could lead to cell cycle arrest.
eIF4E gene transcription is enhanced in response to mitogenic stimulation and may be mediated by c-myc [ 3 ] . Phosphorylation of eIF4E by the protein kinase Mnk1 increases its affinity for the cap, and thus increases cap-dependent initiation of translation [ 4 5 6 ] . An inhibitor of eIF4E, 4E binding protein (4EBP), is also regulated by phosphorylation.
(iii) Lack of interferon response: We provide direct experimental evidence that the 21-nt dsRNA does not trigger an IFN response, which is an advantage because an IFN response would have caused a general and non-specific inhibition of all cap-dependent translation [ 5 ] . (iv) Regulation any time: Although we have not tested this specifically, one can, in principle, transfect the virus-infected cell with dsRNA at any time point during infection.
A novel finding of the experiments above is that synthesis of endogenous p27 Kip1is enhanced under conditions that are expected to reduce cap-dependent translation.
To test this, a 472 nucleotide fragment, representing the full-length human p27 Kip15'-UTR derived from transcription initiation at the major start site, was inserted into the bicistronic expression vector pGL2CAT/Luc [ 30 ] . In this vector, the first cistron, encoding chloramphenicol acetyltransferase (CAT), is proximal to the mRNA's cap structure and therefore expected to be translated by the conventional cap-dependent translation mode.
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