Words similar to calf
Example sentences for: calf
How can you use “calf” in a sentence? Here are some example sentences to help you improve your vocabulary:
For E2F/BrdU double staining, discs were first incubated 30 minutes in a drop of Schneider's medium containing 10% fetal calf serum and 1 mg/ml BrdU then fixed 20 minutes with 2% formaldehyde in PLP (10 mM NaIO 4 , 75 mM Lysine, 37 mM NaH 2 PO 4 , pH 7.2).
To test density effects, cells were plated in duplicate at initial numbers of 1, 2, 5, 10, 20 and 40 × 10 5per 100 mm cell culture dish with DMEM containing 5% FBS and 5% calf serum.
IP 3 measurements were performed on COS-1 cell cytosolic preparations using a radioreceptor assay in which IP 3 and 3H-IP 3 compete for binding to IP 3 receptors prepared from calf cerebellum membranes [ 22 23 ] .
BG-1 cells were maintained in Dulbecco's modified eagle medium (DMEM)/F12 medium supplemented with 10% fetal calf serum (Summit, Fort Collins, CO, USA), and 50 units/ml penicillin/streptomycin.
Medium was added very slowly and consisted of a 1:1 mixture of DMEM and F12 (Life Technologies/Gibco BRL) supplemented with 10% fetal calf serum (Hyclone), 5000 U/ml Penicillin, 53 g/ml Streptomycin (Life Technologies/Gibco BRL), 25 μg/ml L-ascorbic acid (Life Technologies/Gibco BRL) and 10 mM β-Glycerophosphate (Sigma).
Loading...