Example sentences for: calf

How can you use “calf” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • For E2F/BrdU double staining, discs were first incubated 30 minutes in a drop of Schneider's medium containing 10% fetal calf serum and 1 mg/ml BrdU then fixed 20 minutes with 2% formaldehyde in PLP (10 mM NaIO 4 , 75 mM Lysine, 37 mM NaH 2 PO 4 , pH 7.2).

  • Fetal calf serum and RPMI-1640 culture medium supplemented with 25 mM HEPES were from Irvine Scientific.

  • Immortalized cells were maintained in Dulbecco's modified Eagle medium (DMEM, Sigma, St. Louis, MO) with 5% fetal bovine serum (FBS) and 5% calf serum in 5% CO 2 at 37°C.

  • 9% of a constant lot of certified fetal calf serum (FCS; Gibco).

  • Medium was added very slowly and consisted of a 1:1 mixture of DMEM and F12 (Life Technologies/Gibco BRL) supplemented with 10% fetal calf serum (Hyclone), 5000 U/ml Penicillin, 53 g/ml Streptomycin (Life Technologies/Gibco BRL), 25 μg/ml L-ascorbic acid (Life Technologies/Gibco BRL) and 10 mM β-Glycerophosphate (Sigma).


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