Words similar to calf

Example sentences for: calf

How can you use “calf” in a sentence? Here are some example sentences to help you improve your vocabulary:

30–2001) supplemented with 10% fetal calf serum, 10 units/ml penicillin, and 10 μg/ml streptomycin) at 37 °C and 5% CO 2 . For viability studies, cells were seeded in complete growth medium in black 96-well clear bottom ViewPlates (PerkinElmer, Wellesley, Massachusetts, United States) at a density of 5,000 (H1975 and H2030) or 7,500 cells per well (H3255).
The binding reactions were performed in a total volume of 10 μl in a buffer consisting of 10 mM Tris (pH 7.9), 50 mM KCl, 10% glycerol, 50 μg/ml BSA, 1 mM DTT, 10 mM Hepes, 5 mM MgCl 2 , 10 μg/ml calf thymus DNA, and 1.25 fmoles of double-stranded oligonucleotide bearing a high-affinity NFAT site (murine IL-4 promoter from -82 to -64).
HeLa cells were grown in DME/F-12 Ham's 1:1 mix, without phenol red (Sigma) containing 10% iron supplemented calf serum (Sigma) and pen-strep.
After the incubation, an equal volume of DMEM containing 20% calf serum was added for 24 h, bringing the final concentration of calf serum to 10%.
Supplements including: 1% fetal calf serum (GIBCO BRL, Grand Island, NY), 1% l-glutamine (Sigma Chemical), 1% ascorbic acid (Sigma Chemical) and 1% penicillin and streptomycin (Sigma Chemical) were added immediately prior to use.