Example sentences for: calcium-free

How can you use “calcium-free” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • thapsigargin and ryanodine) deplete the ER calcium pool either by (i) inhibiting the ER calcium pump and thereby preventing the replenishment of the ER calcium pool or (ii) triggering ER (ryanodine or inositol 1,4,5-trisphosphate (IP 3 ) activated) calcium release channels [ 9 ] . These phenomena can be visualized by fluorescence microscopy as a transient increase in cytoplasmic calcium concentrations (for example see [ 10 ] ). Nominally calcium-free media are used so that the effect can be distinguished from calcium influx mediated via plasma membrane channels.

  • Magnesium-and calcium-free PBS was used unless noted otherwise in the references.

  • All drugs were prepared in calcium-free Locke's solution containing no EGTA.

  • For calcium-free condition, whole oocytes were rinsed three times with calcium-free frog Ringer's solution (96 mM NaCl, 2 mM KCl, 2 mM EGTA, 1 mM MgCl 2 , 5 mM HEPES, pH 7.6) prior to incubation with or without 10-20 μM capsaicin at room temperature for 30 minutes.


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