Words similar to butait
Example sentences for: butait
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Bioassay driven purification of ButaIT
Considering the high degree of sequence similarity, it is presumed that ButaIT will have a similar 3-D structure to that of other short toxins.
The purified ButaIT was reduced and carboxymethylated by incubating in 6 M guanidine hydrochloride, 0.1 M Tris-HCl (pH 8.3), 1 mM EDTA and 20 mM dithiothreitol for 1 hr at 37°C.
When expressed in baculovirus system as a biopesticide scorpion toxins offer the advantage of faster kill without chemical insecticides [ 13 ] . Although the safety of recombinant baculoviruses is less of a concern ButaIT offers an extra level of safety as a recombinant biopesticide due to its specificity to tobacco budworm ion channels.
Experiments are underway to purify larger quantities of ButaIT using baculovirus expression system in order to establish a refined structure/function relationship.
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