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Example sentences for: bub
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Tem1 is thought to be kept inactive throughout early mitosis by the two-component GTPase-activating protein complex Bub2/Bfa1 which co-localizes with Tem1 at the spindle pole body [ 10 11 ] . When the mitotic spindle penetrates the mother-daughter neck, the Tem1-bound spindle pole body is translocated into the daughter cell where a cortical pool of Lte1 is thought to activate Tem1 [ 12 13 ] . In addition to this spatial regulation, the GTPase activity of Bfa1 is thought to be inactivated through its phosphorylation by Cdc5 [ 14 ] , but it remains unknown how this process is coordinated with the presumed juxtaposition of Lte1 and Tem1 in the daughter cell.
When cells were pre-grown in YPD liquid (instead of SD-URA solid) media before being tested on YPD plates, the percentage of colonies that were red or had red sectors remained similar for wild-type, CDC14 TAB 6-1 , and bub2Δ strains, but was ~25% for net1 tab 2-1.
Because the putative Tem1 antagonists and mitotic checkpoint proteins Bub2 and Bfa1 negatively regulate exit from mitosis and cytokinesis in response to both DNA damage and spindle misorientation ( [ 32 ] ; reviewed in [ 33 ] ), we asked whether bypass of TEM1 -dependent regulation can cause chromosome instability.
The Bub2/Bfa1 GTPase-activating protein (GAP) complex inhibits the MEN and cytokinesis in response to DNA damage and spindle misorientation ( [ 32 ] ; reviewed in [ 33 ] ). bub2Δ and CDC14 TAB 6-1cells show similar chromosome instability as wild-type cells.
Cdc5 presumably impinges on the MEN by other means as well, because Cdc5 is required for maximal Dbf2 protein kinase activity even in the absence of Bfa1's obligate partner Bub2 [ 18 ] . Once activated, the MEN in turn sustains the transient Cdc14 release enabled by Cdc5 and other FEAR pathway components.
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