Words similar to brucellosis
Example sentences for: brucellosis
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Published methods include enterobacterial repetitive intergenic consensus sequence-PCR (ERIC-PCR), and repetitive intergenic palindromic sequence-PCR (REP-PCR) [ 2 3 ] ; random amplified polymorphic DNA-PCR (RAPD-PCR) or arbitrary primed-PCR (AP-PCR) [ 4 5 ] ; and restriction fragment length polymorphism-PCR (RFLP-PCR) of the omp 2 locus [ 6 7 ] . ERIC-PCR and RAPD-PCR are both affected by assay conditions and environmental effects during the amplification process [ 8 9 10 ] . Although the results are highly reproducible within a laboratory, laboratory-to-laboratory reproducibility has been problematic and thus makes the universal application of these methods unlikely (compare [ 2 ] and [ 3 ] ). RFLP-PCR of the omp 2 locus is not constrained by issues of reproducibility and has been useful for the differentiation of Brucella species [ 6 ] and for differentiation among isolates from marine mammal hosts [ 7 ] . However, as a tool for the epidemiology of brucellosis in livestock, this technique is limited by the low rate of natural sequence divergence within the locus at the biovar level.
Most bovine brucellosis in the USA is caused by B. abortus biovar 1. For a typing method to be of value for epidemiological trace-back, it is essential to be able to subtype biovar 1 isolates.
This, in turn, can provide insight into the evolution of a brucellosis outbreak and how to prevent further spread of the disease.
The highest incidence of brucellosis is found in regions where local custom encourages the consumption of raw goat, camel or bovine milk, or of soft cheeses prepared from unpasteurized milk.
However, some regions of the world experiencing high incidence of brucellosis may not have access to the expensive machinery and expertise necessary for fluorescent detection of amplicons.
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