Words similar to brucella
Example sentences for: brucella
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Multiple tandem repeats of the octameric sequence "AGGGCAGT" were initially discovered when searching sequence files generated from the shotgun sequencing of Brucella abortus biovar 1, strain 9-941 for a subsequence (TAGGGC) of IS 711 . The B. abortus genome had nine chromosomal loci containing at least two complete copies of the octameric repeat.
Historically, it has been very difficult to irrefutably trace natural Brucella transmission unless the strain involved happened to belong to a rare biovar.
Published methods include enterobacterial repetitive intergenic consensus sequence-PCR (ERIC-PCR), and repetitive intergenic palindromic sequence-PCR (REP-PCR) [ 2 3 ] ; random amplified polymorphic DNA-PCR (RAPD-PCR) or arbitrary primed-PCR (AP-PCR) [ 4 5 ] ; and restriction fragment length polymorphism-PCR (RFLP-PCR) of the omp 2 locus [ 6 7 ] . ERIC-PCR and RAPD-PCR are both affected by assay conditions and environmental effects during the amplification process [ 8 9 10 ] . Although the results are highly reproducible within a laboratory, laboratory-to-laboratory reproducibility has been problematic and thus makes the universal application of these methods unlikely (compare [ 2 ] and [ 3 ] ). RFLP-PCR of the omp 2 locus is not constrained by issues of reproducibility and has been useful for the differentiation of Brucella species [ 6 ] and for differentiation among isolates from marine mammal hosts [ 7 ] . However, as a tool for the epidemiology of brucellosis in livestock, this technique is limited by the low rate of natural sequence divergence within the locus at the biovar level.
We anticipate that the testing of a larger number of isolates from various geographic areas will ultimately lead to the development of an international Brucella fingerprint database that can be searched for epidemiological use.
The assay is intended to complement the existing PCR and bacteriological diagnostic tests currently used for Brucella species and biovar identification.
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