Words similar to brca

Example sentences for: brca

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The primers used for amplification of methylated and unmethylated promoters of genes RARB , TIMP3 , CDKN2A , p14 ARF , MGMT , DAPK , CDH1 , GSTP1 , APC promoter 1A, RB1 , MLH1 , TP73 , BRCA1 , FHIT , and HIC1 have been described previously http://pathology2.jhu.edu/pancreas/prim0425.htm#MSP; [ 32 47 48 49 ] . Methylated (MF and MR) and unmethylated (UF and UR) primers for RASSF1A promoter were designed according to Herman et al [ 46 ] and as described earlier [ 50 ] . PCR products were run on 2% agarose gels and visualized after ethidium bromide staining.
Clones were isolated from the BRCA1 antisense and LXSN BG-1 pooled populations and grown in phenol red-free, DMEM/F-12 medium (Gibco/Life Technologies) supplemented with charcoal/dextran treated serum (Hyclone) for 5 days before plating for growth curve analysis.
A BRCA1 ribonuclease protection probe was made using an In Vitro Transcription Kit (Ambion, Inc, Austin, TX, USA).
This hypothesis is based on the fact that two regions on chromosome 17q are sometimes amplified in breast cancer, one at 17q11-21 and another at 17q22-24 [ 34 35 ] . The locus of BP1 is also near BRCA1; the implications of this proximity are not yet known.
All BRCA1 antisense subclones were able to proliferate as well as exhibit a decreased death rate in estrogen-deprived media, whereas parental and control subclones failed to grow.