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Example sentences for: brca

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Subclones were also isolated from BRCA1 antisense infected cells, or LXSN infected cells (NEO).
A partial human cDNA sequence of BRCA1 (from nucleotide 121 in exon 1 to nucleotide 1025 in exon 11, accession #U14680) was inserted in the antisense orientation into the EcoR1 site of the pLXSN retroviral vector (provided by AD Miller).
First, to determine whether a mutation in amino-terminal RING domain of BRCA1 preferentially targeted either the mitochondrial [ 25 ] or Fas/Fas ligand apoptotic pathway [ 24 ] , levels of the respective initiator caspases 9 and 8 were determined.
The vector alone, or the antisense BRCA1 vector, was transfected using the calcium-phosphate precipitation method into the ecotropic packaging cell line GPE86 [ 31].
BRCA1 has been shown to colocalize in nuclear dots with other cellular proteins, including BARD-1 [ 24], Rad51, PCNA, and BRCA2 [ 7, 25].