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Example sentences for: boehringer-

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Subsequently, the sections were rinsed 4 times with .1 M PBS + .2% Triton-X and incubated for 1 hour at room temperature with the secondary antibody (FITC-conjugated sheep anti-mouse IgG; Boehringer-Mannheim, Indianapolis, IN; Cat.
The cryo-sections were air-dried at room temperature, fixed in acetone for 5 min., washed for 15 min in Tris-buffered saline (TBS; 20 mM Tris-HCl, 500 mM NaCl, pH 7.4) and incubated for 1 hour with 1% purified milk protein blocking solution (Boehringer-Mannheim, Indianapolis, IN) in TBS to reduce background staining.
Detailed procedures for isolation of total RNA, enrichment of poly (A)+ RNA by oligo-dT cellulose chromatography and electrophoresis and Northern blotting of RNA have been described previously [ 13 ] . The cDNA probes of mouse RAR α and RAR γ [ 21 22 ] were isolated by agarose electrophoresis after appropriate endonucleases digestion and were radio-labeled with 32P-dCTP, using random priming kit (Boehringer-Mannheim, Indianapolis, IN).
Apoptosis was assessed by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate (dUTP) nick end labeling (TUNEL) (In situ Cell Death Detection Kit, POD, Boehringer-Manheim).
The following day, the sections were rinsed again and incubated for 30 minutes at room temperature with FITC-conjugated sheep anti-mouse IgG (Boehringer-Mannheim; diluted 1:100).