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Example sentences for: boehringer

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The second antibodies were a 1:100 dilution of FITC-labeled goat anti-human antibodies (Waldeim Pharmazeutika) and a 1:50 dilution of goat anti-mouse IgG-L-Rhodamine (Boehringer Mannheim Biochemicals).
1 M maleic acid, 3 M NaCl, 0.3% Tween 20, pH 8) at room temperature followed by blocking for two hours at room temperature in maleate buffer with 0.5% blocking reagent (Boehringer Mannheim, Biochemical Products, Indianapolis IN).
Antibodies used were directed against the His epitope (monoclonal, Boehringer Mannheim), SecB (polyclonal, gift from William Wickner), or LamB (polyclonal, gift of Thomas Silhavy).
Briefly, the superior cervical ganglia were dissected from adult rats and incubated in Earle's balanced salt solution (Life Technologies, Rochelle, MD) containing 0.45 mg ml -1trypsin (Worthington, Freehold, NJ), 0.6 mg ml -1collagenase D (Boehringer Mannheim, Indianapolis, IN) and 0.05 mg ml -1DNAse I (Sigma, St. Louis, MO) for 1 hour at 35°C.
Antibodies against the following proteins were obtained from the following suppliers or individuals: ant-phosphotyrosine, RC20-HRP (Transduction Laboratories, Lexington, KY); anti-HA (Boehringer Mannheim, Indianapolis, IN); anti-gag (Dr.