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Example sentences for: blebs

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In isolated bovine aortic endothelial cells (BAECs), the opening or activation of COP is associated with formation of spherical membrane blebs with a diameter of 3-5 microns [ 3 ] . The final stage of MTX-induced cell death is cell lysis as indicated by the release of large cytoplasmic enzymes, such as lactate dehydrogenase (LDH).
The second major conclusion supported by the results of the present study is that MTX apparently has an effect on BAECs that is independent of the rise in [Ca 2+] i . We previously showed that MTX causes the appearance of membrane blebs on the surface of the endothelial cells [ 3 ] . Initially these blebs are of small diameter (3-5 microns) and bleb formation correlates with the first phase of vital dye uptake.
, a cell death cascade) that is triggered by the activation of CaNSC and a rise in [Ca 2+] i . This is followed by formation or activation of COP which is correlated with the formation of membrane blebs.
However, blebbing presumably requires a change in osmotic pressure within the cell and so we reasoned that the blebs may be associated with the second phase of vital dye uptake.
, 20 min after addition of MTX) large membrane blebs were observed on essentially all the cells within the field of view (Fig 5A, see examples indicated by blue arrows ). Additionally, clear staining of the nucleus and cytoplasm by ethidium was observed at this time.