Words similar to blasticidin

Example sentences for: blasticidin

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All four showed an expected 1 kb shift compared with the parental strain, Ax4, resulting from the presence of the blasticidin resistance cassette (Fig.
A plasmid was constructed that carried 825 bp from the pseudokinase domain of ifkA and 731 bp from the kinase domain, placed at either end of a 1.4 kb blasticidin resistance cassette [ 48 ] . A 3.95 kb EcoR I/ Hind III fragment, containing the ifkA sequences and the blasticidin gene, was released from the plasmid and it's ends were made blunt using the klenow fragment and dephosphorylated to prevent recircularization and reduce nonspecific integration of the fragment.
As only the 3' flanking sequence of the blasticidin resistance cassette is found in ifkB but not the 5' flanking sequence, the disruption construct should be specific for
The blasticidin resistance cassette replaced a 391 bp fragment that includes the first four subdomains (the ATP binding site) of the kinase domain.
Several independent blasticidin resistant colonies were obtained and were initially screened using PCR.